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In vitro culture of wirrzopf tissue of Salix alba L

In vitro culture of wirrzopf tissue of Salix alba L

Journal of Plant Physiology 145(1-2): 178-180

Wirrzopf organoid gall tissue of Salix alba was analysed by in vitro culture. The tissue maintained its abnormal growth pattern for more than 2.5 years on a modified ACM medium (Ahuja, 1983) with 1.11 mu-M BAP. On hormone-free medium this growth pattern was also maintained, although the growth rate was reduced. Formation of thin, threadlike shoots was induced by the addition of GA-3 (2.9-57.8 mu-M) and such shoots were rooted by the addition of IBA (50 mu-M). Rooted shoots died sooner or later, which corresponds to the limited life expectancy of wirrzopf galls in planta.

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Accession: 008836813

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DOI: 10.1016/s0176-1617(11)81867-5

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