EurekaMag.com logo
+ Site Statistics
References:
53,517,315
Abstracts:
29,339,501
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

In vitro culture of primary plasmacytomas requires stromal cell feeder layers



In vitro culture of primary plasmacytomas requires stromal cell feeder layers



Proceedings of the National Academy of Sciences of the United States of America 90(5): 2060-2064



Attempts to grow primary murine plasmacytomas in vitro have, to date, been largely unsuccessful. In this study, we demonstrate that long-term in vitro growth of primary plasmacytomas is accomplished by using feeder layers composed of stromal cells from the initial site of plasmacyto magenesis. The early neoplastic lines established in this manner are dependent on physical contact with the stromal layer, which is mediated in part by CD44, for growth and survival. The stromal cells provide at least two stimuli for the plasma cells, one being interleukin 6 and the second, of unknown nature, resulting from direct physical interaction that cannot be replaced by soluble factors. These plasma cell lines have been passaged for as long as 20 months yet still maintain characteristic associated with primary plasmacytomas as they will grow in vivo only in pristane-primed animals, indicating a continued dependence on the pristane-induced microenvironment characteristic of early-stage tumors. The ability to grow primary plasmacytomas in culture and maintain their "primary" properties provides a model system for detailed analysis of early events in plasma cell tumor progression involving neoplastic cells completely dependent on physical contact with a stromal feeder layer for survival and expansion.

(PDF same-day service: $19.90)

Accession: 008836862

Download citation: RISBibTeXText

PMID: 8446628

DOI: 10.1073/pnas.90.5.2060



Related references

Effects of different feeder layers on culture of bovine embryonic stem cell-like cells in vitro. Cytotechnology 66(6): 995-1005, 2014

Studies On The Action Of Feeder Layers In Cell Culture. Experimental Cell Research 38: 645-653, 1965

Maintaining Growth of Long-Term Culture Initiating Cells from Human Cord Blood on Feeder Layers of Bone Marrow Stromal Cells Transfected with FL and/or TPO Genes. Zhongguo Shi Yan Xue Ye Xue Za Zhi 9(2): 97-100, 2003

Middle-term expansion of hematopoietic cord blood cells with new human stromal cell line feeder-layers and different cytokine cocktails. International Journal of Molecular Medicine 24(6): 837-845, 2010

Effects of different feeder layers on short-term culture of prepubertal bovine testicular germ cells in-vitro. Theriogenology 77(8): 1519-1528, 2013

In vitro development of bovine one-cell embryos fertilized in vitro in serum- and feeder cell-free culture systems. Asian-Australasian Journal of Animal Sciences 9(5): 583-590, 1996

In vitro cultivation of blood stream trypomastigotes of Trypanosoma vivax without feeder cell layers. Journal of Protozoology Research 1(15): 1-12, 1991

In vitro cultivation of Trypanosoma congolense bloodstream forms in the absence of feeder cell layers. Parasitology 102 Pt 2: 225-236, 1991

Growth on marrow stromal feeder layers of blast cell colonies derived from primitive progenitor cells circulating in chronic granulocytic leukemia in chronic phase. British Journal of Haematology 63(1): 203, 1986

Quantitation of cell proliferation, colony formation, and carcinogen induced cytotoxicity of rat tracheal epithelial cells grown in culture on 3T3 feeder layers. In Vitro 19(7): 559-570, 1983

The role of the BMP axis in stem cell expansion Changes in BMP4 production by primitive cord blood cells following co-culture with the stromal feeder AFT024. Experimental Hematology (New York) 31(7 Supplement 1): 197, July, 2003

The mouse fetal liver cell line, AFT024, supports human myelopoiesis and lymphopoiesis better than primary stroma, but this requires contact with the feeder. Blood 88(10 SUPPL 1 PART 1-2): 535A, 1996

Effects of Feeder Cell Types on Culture of Mouse Embryonic Stem Cell In Vitro. Development & Reproduction 19(3): 119-126, 2016

Feeder layers: co-culture with nonneoplastic cells. Methods in Molecular Biology 731: 467-470, 2011

Transgenic mice for the preparation of hygromycin-resistant primary embryonic fibroblast feeder layers for embryonic stem cell selections. Nucleic Acids Research 23(7): 1273-1275, 1995