EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

In vitro production of type 1 and type 2 cytokines by peripheral blood mononuclear cells from high-risk HIV-negative intravenous drug users


In vitro production of type 1 and type 2 cytokines by peripheral blood mononuclear cells from high-risk HIV-negative intravenous drug users



Aids 9(7): 691-694



ISSN/ISBN: 0269-9370

PMID: 7546412

DOI: 10.1097/00002030-199507000-00004

Objective: To study type 1 and type 2 cytokine patterns in HIV-negative high-risk intravenous drug users (IVDU). Design: We investigated interleukin (IL)-2, interferon (IFN)-gamma, IL-4, IL-6 and IL-10 production by phytohaemagglutinin (PHA)-stimulated and unstimulated peripheral blood mononuclear cell (PBMC) cultures from HIV-negative high-risk IVDU, HIV-negative controls and HIV-positive subjects. Methods: Cytokine production was measured in supernatants using enzyme-linked immunosorbent assay (ELISA) in 10 HIV-negative high-risk IVDU, 25 HIV-negative controls, and 12 HIV-positive IVDU. We also determined spontaneous in vitro immunoglobulin (Ig) G and IgM production. Results: HIV-negative high-risk IVDU showed increased IFN-gamma and decreased IL-4, IL-10 and IL-2, although the latter was not significant compared with HIV-negative controls. Further, HIV-negative high-risk IVDU had reduced IgG production and impaired IgM-IgG switch. Conclusions: The reduced IL-2 and IL-4 production suggest an impaired CD4+ T-cell function in HIV-negative high-risk IVDU. The increased IFN-gamma production along with the decreased type 2 cytokine profile is consistent with the hypothesis that protective immunity against HIV may reside in type 1 responses and cell-mediated immunity.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008839060

Download citation: RISBibTeXText

Related references

In vitro production of type 1 and type 2 cytokines by peripheral blood mononuclear cells from subjects coinfected with human immunodeficiency virus and Leishmania infantum. American Journal of Tropical Medicine and Hygiene 60(1): 142-145, 1999

Production of type-1 and type-2 cytokines by peripheral blood mononuclear cells of psoriatic patients. Immunology 86(3): 422-426, 1995

Acetylcholine receptor stimulation of peripheral blood mononuclear cells leads to production of TH-2 type cytokines. 9TH INTERNATIONAL CONGRESS OF IMMUNOLOGY Author The 9th International Congress of Immunology : 640, 1995

In vitro TNF-alpha and IL-6 production by adherent peripheral blood mononuclear cells obtained from type 1 and type 2 diabetic patients evaluated according to the metabolic control. Annals of the new York Academy of Sciences 1079: 177-180, 2006

Detection of TTV in peripheral blood mononuclear cells of intravenous drug users. Tohoku Journal of Experimental Medicine 188(1): 47-54, 1999

Insulin increases in vitro production of Th2 profile cytokines in peripheral blood cultures in subjects at high risk of diabetes type 1 and patients with newly diagnosed IDDM. Hormone and Metabolic Research 31(4): 289-292, 1999

Effect of anti-tumor arabinomannan extracted from M tuberculosis on the production of IL-12 by peripheral blood mononuclear cells treated with type 2 cytokines. Proceedings of the American Association for Cancer Research Annual Meeting 39: 532, 1998

Short communication: human lymphotropic virus type 1 coinfection modulates the synthesis of cytokines by peripheral blood mononuclear cells from HIV type 1-infected individuals. Aids Research and Human Retroviruses 28(8): 806-808, 2012

Immunostimulatory DNA activates production of type I interferons and interleukin-6 in equine peripheral blood mononuclear cells in vitro. Veterinary Immunology and Immunopathology 107(3-4): 265-279, 2005

High interleukin-12 production from stimulated peripheral blood mononuclear cells of type 2 diabetes patients. Cytokine 51(3): 298-304, 2010

Effect of metabolic control on the in vitro proliferation of peripheral blood mononuclear cells in type 1 and type 2 diabetic patients. Sao Paulo Medical Journal 124(4): 219-222, 2006

DHMEQ, a novel nuclear factor-kappaB inhibitor, induces selective depletion of alloreactive or phytohaemagglutinin-stimulated peripheral blood mononuclear cells, decreases production of T helper type 1 cytokines, and blocks maturation of dendritic cells. Immunology 124(2): 198-205, 2008

In vitro production of cytokines by peripheral blood mononuclear cells from hydatid patients. Clinical and Experimental Immunology 99(3): 433-439, 1995

Effect of the glycemic control on intracellular cytokine production from peripheral blood mononuclear cells of type 1 and type 2 diabetic patients. Diabetes Research and Clinical Practice 82(3): 329-334, 2008

Differential effects of propentofylline on the production of cytokines by peripheral blood mononuclear cells in vitro. Clinical Therapeutics 13(6): 747-753, 1991