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Inactivation of Bacillus spores by the supercritical carbon dioxide micro-bubble method

Inactivation of Bacillus spores by the supercritical carbon dioxide micro-bubble method

Bioscience Biotechnology and Biochemistry 61(6): 1022-1023

ISSN/ISBN: 0916-8451

PMID: 9214765

DOI: 10.1271/bbb.61.1022

Bacillus spores were effectively inactivated by the supercritical (SC) CO2 micro-bubble method. The micro-bubble SC CO2 treatment of B. cereus, B. subtilis, B. megaterium, B. polymyxa, and B. coagulans at 40 degrees C and 30 MPa for 30 min produced greater reduction (about 3 log cycles of reduction) than a similar treatment without a filter. The SC CO2 treatment of B. polymyxa, B. cereus, and B. subtilis spores at 45 degrees C, 50 degrees C, respectively, and 30 MPa for 60 min resulted in a 6-log cycle reduction of survival. The SC CO2 treatment under the foregoing conditions should offer higher efficiency than that of heat treatment at 100 degrees C for 60 min. In addition, the SC CO2 treatment (30 MPa, 60 degrees C, 30 min) of B. polymyxa and B. cereus spores also produced a 6-log cycle reduction.

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Accession: 008843925

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