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Inactivation of Enzymes in an Aqueous Solution by Micro-bubbles of Supercritical Carbon Dioxide


Inactivation of Enzymes in an Aqueous Solution by Micro-bubbles of Supercritical Carbon Dioxide



Bioscience Biotechnology and Biochemistry 59(4): 628-631



ISSN/ISBN: 0916-8451

DOI: 10.1271/bbb.59.628

Enzyme solutions of glucoamylase, acid protease, alkaline protease and lipase were treated with micro-bubbles of supercritical carbon dioxide (SC CO-2) fed from a cylindrical filter nozzle. The microbubbles of SC CO-2 could increase the CO-2 concentration in the sample solution from 0.4 to 0.92 mol/l at 25 MPa and 35 degree C, and hence could improve the efficiency of inactivation by about 3 times compared to treating without the filter nozzle. Alkaline protease and lipase in the solution could be completely inactivated by the treatment at 35 degree C and 15 MPa for 30 min. With the inactivation of glucoamylase and acid protease, their residual activity-CO-2 density profiles consisted of two straight lines with intersections at density values of 0.82 and 0.60 g/cm-3, respectively. These enzymes showed an abrupt decrease in activation in regions above the CO-2 density of their intersections.

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Accession: 008843951

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