Incidence of chromosome 18 disomy in human sperm nuclei as detected by nonisotopic in situ hybridization

Guttenbach, M.; Schakowski, R.; Schmid, M.

Human Genetics 93(4): 421-423

1994


ISSN/ISBN: 0340-6717
PMID: 8168812
DOI: 10.1007/bf00201667
Accession: 008845472

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Abstract
Nonradioactive in situ hybridization with an alpha-satellite DNA probe specific for chromosome 18 was performed on human interphase sperm nuclei to detect the frequency of sperm cells disomic for chromosome 18. A total of 16127 sperm heads from eight healthy donors, aged 23-57 years, was investigated, and a minimum of 2000 sperm nuclei per proband was analyzed. The disomy rate ranged from 0.25% to 0.5%, with an average of 0.36%. This frequency does not differ significantly from that determined for other chromosomes.

Incidence of chromosome 18 disomy in human sperm nuclei as detected by nonisotopic in situ hybridization