+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Increase in beta-galactosidase activity in a non-isothermal bioreactor utilizing immobilized cells of Kluyveromyces fragilis: fundamentals and applications

Increase in beta-galactosidase activity in a non-isothermal bioreactor utilizing immobilized cells of Kluyveromyces fragilis: fundamentals and applications

Research in Microbiology 148(3): 271-281

The beta-galactosidase activity of Kluyveromyces fragilis cells immobilized in a kappa carrageenan gel was studied in a bioreactor functioning under isothermal and non-isothermal conditions. We observed an increase in enzyme activity which was found to be proportional to the intensity of the temperature gradient applied across the biocatalytic membrane, as well as to the average temperature of the bioreactor. The efficiency of such a non-isothermal bioreactor was calculated with respect to the yield of a bioreactor working under comparable isothermal conditions and was evaluated in terms of reduction of processing times in industrial applications. The possibility that enzyme activity in living cells is affected by non-isothermal conditions naturally existing owing to metabolic heat production is also discussed.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 008847090

Download citation: RISBibTeXText

PMID: 9765807

DOI: 10.1016/s0923-2508(97)85247-3

Related references

Hydrolysis of lactose in whey permeate by immobilized beta-galactosidase from Kluyveromyces fragilis. Journal of Molecular Catalysis B Enzymatic 10(6): 631-637, 1 November, 2000

Kinetic modeling of lactose hydrolysis with an immobilized beta-galactosidase from Kluyveromyces fragilis. Enzyme and Microbial Technology 27(8): 583-592, 2000

A non-isothermal bioreactor utilizing immobilized baker's-yeast cells: a study of the effect on invertase activity. Biotechnology and Applied Biochemistry 23: 141-148, 1996

Comparison between immobilized Kluyveromyces fragilis and Saccharomyces cerevisiae coimmobilized with beta-galactosidase, with respect to continuous ethanol production from concentrated whey permeate. Biotechnology and Bioengineering 27(6): 914-916, 1985

Influence of spray drying on the permeability of kluyveromyces fragilis measured by beta galactosidase activity. Food Biotechnology (New York) 6(2): 135-152, 1992

Bioreactor immobilized enzymes and cells fundamentals and applications. Moo-Young, M (Ed ) Bioreactor Immobilized Enzymes And Cells: Fundamentals And Applications Xvi+327p Elsevier Science Publishers Ltd : Essex, England, Uk; New York, New York, Usa Illus Xvi+327p, 1988

Effects of temperature and pH on the catalytic activity of the immobilized beta -galactosidase from Kluyveromyces lactis. Biochemical Engineering Journal 9(1): 33-40, 2001

Immobilized-enzyme activity under isothermal and non-isothermal conditions: Studies on beta-galactosidase as a model enzyme. Biotechnology and Applied Biochemistry 22(3): 281-294, 1995

Hydrolysis of lactose in whole milk catalyzed by β-galactosidase from Kluyveromyces fragilis immobilized on chitosan-based matrix. Biochemical Engineering Journal 81: 54-64, 2013

Immobilized beta galactosidase ec on insoluble carrier part 2 technological applications of immobilized beta galactosidase. Lebensmittel-Wissenschaft & Technologie 8(6): 278-281, 1975

Immobilization and characterization of kluyveromyces fragilis beta galactosidase. Indian Journal of Experimental Biology 18(9): 1020-1023, 1980

Studies on beta -galactosidase production by Kluyveromyces fragilis. Indian Journal of Dairy Science 37(2): 133-136, 1984

Isolation of a Kluyveromyces fragilis gene coding for beta-galactosidase. Biotecnologia Aplicada 12(1): 42-45, 1995

Production of beta-galactosidase from Kluyveromyces fragilis grown on whey. Folia Microbiologica 26(5): 370-376, 1981

Immobilization of beta galactosidase from kluyveromyces fragilis on ion exchange resin. Australian Journal of Dairy Technology 42(3-4): 62-64, 1987