Influence of cadmium ions on endothelin-1 binding and calcium signaling in rat glioma C6 cells

Koschel, K.; Meissner, N.N.; Tas, P.W.

Toxicology Letters 81(2-3): 189-195

1995


ISSN/ISBN: 0378-4274
PMID: 8553374
DOI: 10.1016/0378-4274(95)03434-x
Accession: 008864290

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Abstract
Cadmium ions did not influence the binding of endothelin-1 (ET-1) to its receptor on the surface of rat glioma C6 and rat aorta A10 cells. This was studied (a) by the binding of 1251-ET-1 to intact cells in the absence or presence of cadmium (Cd2+) and (b) by analysis of the receptor/ET-1 complex after crosslinking with disuccinimidyl suberate (DSS) or ethylene glycol-bis-(succinimidyl succinate) (EGS) on SDS PAGE. Using Fura-2 and Quin-2 loaded C6 rat glioma cells, it was shown that Cd2+ ions strongly interfered with the ET-1 induced Ca(2+)-influx in C6 glioma cells (IC50 approximately 10 microM).