Section 9
Chapter 8,867

Influence of extraction medium on Bois noir and Flavescence doree detection, using polyclonal and monoclonal antibodies against stolbur and Flavescence doree phytoplasmas

Kuszala, C.

Agronomie 16(6): 355-365


ISSN/ISBN: 0249-5627
Accession: 008866086

A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) led us to show that a monoclonal antibody, specific for stolbur phytoplasma, recognized a 'Bois noir'-associated agent in extracts of diseased grapevines from different countries (France, Italy and Switzerland). In addition, we developed a common extraction procedure for the ELISA identification of both stolbur and Flavescence doree phytoplasmas.

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