EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Inhalant allergens in asthmatic children in Taiwan: comparison evaluation of skin testing, radioallergosorbent test and multiple allergosorbent chemiluminescent assay for specific IgE


Inhalant allergens in asthmatic children in Taiwan: comparison evaluation of skin testing, radioallergosorbent test and multiple allergosorbent chemiluminescent assay for specific IgE



Journal of the Formosan Medical Association 91(12): 1127-1132



ISSN/ISBN: 0929-6646

PMID: 1363631

The multiple allergosorbent chemiluminescent assay (MAST-CLA) is a method for measuring total and allergen-specific IgE in human serum by a chemiluminescent immuno-enzymatic system. To compare the results of MAST tests with those of radioallergosorbent (RAST) and skin tests as an adjunct to the diagnosis of inhalant allergens in Taiwan, 195 asthmatic children, aged 5 to 15 years, were studied. All MAST tests had valid positive and negative control threads. The most important allergens in our patients were the two mite species: Dermatophagoid pteronyssinus and Dermatophagoid farinae (87.5% and 82.1%, respectively). There were also large responses (MAST-CLA class > or = 2) to the cockroach mix (28.45%), Alternaria (24.3%), and Eucalyptus (32.6%). The individual efficiency percentage between MAST-CLA and skin tests was D. pteronyssinus 91%, D. farinae 88%, cockroach mix 85%, feather mix 75%, dog dander 64%, Candida 75%, Aspergillus 79%, Alternaria 88%, ragweed mix 62% and Eucalyptus 76%. Comparison of the results of allergen-specific IgE measured by MAST-CLA and RAST were also significantly correlated for all four allergens (D. farinae, Candida, Alternaria and grass mix, r = 0.79-0.92). MAST-CLA was randomly duplicated and proven reproducible in 89% of the tests. Changes between positive and negative results occurred in only 3.6% of the tests. MAST-CLA is a simple in vitro test for specific IgE to 35 allergens, which compares favorably with RAST. It is concluded that MAST-CLA and RAST are similar in their ability to measure allergen-specific IgE, and correlate equally well with skin tests and clinical history in asthmatic children.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 008873891

Download citation: RISBibTeXText

Related references

Inhalant allergy in asthmatic children: skin prick test, radioallergosorbent test and chemiluminescent assay compared with allergen levels in their mattress dusts. International Archives of Allergy and Applied Immunology 88(1-2): 183-184, 1989

Inhalant Allergy in Asthmatic Children: Skin Prick Test, Radioallergosorbent Test and Chemiluminescent Assay Compared with Allergen Levels in Their Mattress Dusts. International Archives of Allergy and Immunology 88(1-2): 183-184, 1989

Comparative evaluation of the multiple radioallergosorbent test and discrete radioallergosorbent test for inhalant allergy. Allergy 41(5): 327-330, 1986

Comparison of fluorescent allergosorbent test radioallergosorbent test prick testing in highly allergic individuals. Annals of Allergy 56(6): 521, 1986

A comparative study of immediate skin test reactivity to inhalant allergens in asthmatic children of two different regions in Saudi Arabia. Annals of Saudi Medicine 12(5): 468-471, 1992

Evaluation of the specific activity of commercial preparations of house-dust allergens in the bacteriosorbent test: comparison with results of the radioallergosorbent inhibition test. Zhurnal Mikrobiologii, Epidemiologii i Immunobiologii (9): 69-73, 1986

Two screening methods for detection of specific IgE to inhalant allergens. Comparison with skin prick test and RAST. Journal of Investigational Allergology and Clinical Immunology 1(5): 324-329, 1991

Skin-prick test reactions to inhalant allergens in asthmatic patients. Medical Journal of Australia 1(24): 918-924, 1976

Inhalant and food allergens in atopic eczema assessed by radio allergosorbent test studies. Macdonald, D M (Ed ) Immunodermatology; Symposium; London, England, Sept 1983 Xix+291p Butterworths: Stoneham, Mass , Usa; London, England Illus 13-16, 1984

Evaluation of sensitivity and specificity of skin prick test in correlation with specific ige for nine inhalant allergens in kuwait. Annals of Allergy 60(2): 176, 1988

Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children. Annals of Allergy 62(5): 432-435, 1989

Comparison of serum-specific IgE (ImmunoCAP) and skin-prick test results for 53 inhalant allergens in patients with chronic rhinitis. Allergy and Asthma Proceedings 30(4): 386-396, 2009

Relationships between skin prick test, radioallergosorbent test, and chemiluminescent assays in allergic children. Annals of Allergy 66(2): 137-141, 1991

Results of skin tests to inhalant allergens in asthmatic children vs healthy controls. Journal of Allergy and Clinical Immunology 103(1 Part 2): S17, 1999

Dermatophagoides farinae and Japanese cedar (sugi) pollen specific immunoglobuline E antibodies in asthmatic children measured by radioallergosorbent test and enzyme-linked immunosorbent assay. Arerugi 38(6): 451-458, 1989