EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Inhibition of chick embryo hepatic uroporphyrinogen decarboxylase by components of xenobiotic-treated chick embryo hepatocytes in culture: II



Inhibition of chick embryo hepatic uroporphyrinogen decarboxylase by components of xenobiotic-treated chick embryo hepatocytes in culture: II



Canadian Journal of Physiology and Pharmacology 70(7): 939-942



A variety of xenobiotics, viz., 3,3',4,4'-tetrachlorobiphenyl (TCBP), sodium phenobarbital (PB), 3,5-diethoxycarbonyl-2, 4,6-trimethylpyridine (OX-DDC), and nifedipine, cause a decrease in uroporphyrinogen decarboxylase (UROG-D) activity, accompanied by uroporphyrin accumulation, in chick embryo hepatocytes in culture. In this study the activity of 17-day-old chick embryo hepatic UROG-D was determined by measuring the conversion of pentacarboxylporphyrinogen I to coproporphyrinogen I, and it was shown that a UROG-D inhibitor, previously reported to accumulate in TCBP-treated and PB-treated chick embryo hepatocytes in culture, also accumulates in OX-DDC-treated and nifedipine-treated chick embryo hepatocytes in culture. It was concluded that the accumulation of a UROG-D inhibitor provides an explanation for the UROG-D inhibition observed in this culture system with xenobiotics that cause uroporphyrin accumulation. Studies of the UROG-D inhibitory fraction isolated from the 10,000 x g, 40,000 x g, and 100,000 x g supernatant fractions of cultured chick embryo hepatocyte homogenate led to the conclusion that the UROG-D inhibitor is derived from a soluble component of the homogenate.

(PDF emailed within 0-6 h: $19.90)

Accession: 008877234

Download citation: RISBibTeXText

PMID: 1451030

DOI: 10.1139/y92-128



Related references

Inhibition of chick embryo hepatic uroporphyrinogen decarboxylase by components of xenobiotic-treated chick embryo hepatocytes in culture. Canadian Journal of Physiology and Pharmacology 67(3): 246-249, 1989

Xenobiotic mediated inhibition of hepatic uroporphyrinogen decarboxylase activity in 17-day-old chick embryo liver cells in culture. Biochemical Pharmacology 37(6): 1123-1129, 1988

Inhibition of uroporphyrinogen decarboxylase by 3,3',4,4'-tetrachlorobiphenyl in chick embryo liver cell culture. Canadian Journal of Physiology and Pharmacology 61(1): 105-108, 1983

Hepatic uroporphyrin accumulation and uroporphyrinogen decarboxylase activity in cultured chick embryo hepatocytes and in japanese quail coturnix coturnix japonica and mice treated with polyhalogenated aromatic compounds. Biochemical Journal 253(1): 131-138, 1988

Comparison of uroporphyrinogen decarboxylase activities and uroporphyrin accumulation in mice japanese quail and cultured chick embryo hepatocytes treated with polyhalogenated aromatic compounds. Annals of the New York Academy of Sciences 514(none): 337-338, 1987

Polychlorinated aromatic hydrocarbon lethality, mixed-function oxidase induction, and uroporphyrinogen decarboxylase inhibition in the chick embryo: dissociation of dose-response relationships. Toxicology and Applied Pharmacology 78(2): 268-279, 1985

Experimental infection of chick embryo fibroblast cell culture and chick embryo trachea organ culture with mycoplasma gallisepticum strain s 6. Sudan Journal of Veterinary Science & Animal Husbandry 14(2): 56-64, 1973

Data on epidemic parotiditis. I. Biology of local strains in chick embryo culture; isolation & adaptation to chick embryo. Prensa Medica Argentina 44(5): 339-347, 1957

Effects of Sephadex fractions of chick embryo extract on growth and differentiation of endothelial cells from chick embryo hearts in culture. Mie Medical Journal 19(1): 29-40, 1969

Action of heparin on the neural tube of the chick embryo. New technic of chick embryo culture in vitro. Revista de la Sociedad Argentina de Biologia 41(1): 19-23, 1965

Inhibition of uro porphyrinogen decarboxylase ec 41137 by 3 3 4 4 tetra chloro bi phenyl in chick embryo liver cell culture. 1983

Inhibition of heparan sulfate biosynthesis in cultures of chick embryo fibroblasts by tunicamycin and formation of lipid-linked glucuronic acid in chick embryo microsomes. Agricultural and Biological Chemistry 42(8): 1621-1623, 1978

Inhibition of heparan sulfate biosynthesis in cultures of chick embryo fibroblasts by tunicamycin and formation of lipid linked glucuronic acid in chick embryo microsomes. Agricultural and Biological Chemistry 42(8): 1621-1624, 1978

Polychlorobiphenyls that induce delta-aminolevulinic acid synthetase inhibit uroporphyrinogen decarboxylase in cultured chick embryo liver cells. Febs Letters 129(1): 93-96, 1981

Study of some enzyme activities in cultured chick embryo brain nerve cells treated by chick embryo brain extracts. Neurochemical Research 5(8): 831-845, 1980