+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Inhibition of transcription starting from bacteriophage lambda pR promoter during the stringent response in Escherichia coli: implications for lambda DNA replication

Inhibition of transcription starting from bacteriophage lambda pR promoter during the stringent response in Escherichia coli: implications for lambda DNA replication

Acta Biochimica Polonica 42(2): 233-239

Replication of lambda plasmid DNA is halted in amino acid-starved wild type (stringent) strains whereas it proceeds in relA (relaxed) mutants. The only transcription which could be important in lambda plasmid DNA replication in amino acid-starved Escherichia coli cells is that starting from the PR promoter. Using a fusion which consists of the lacZ gene under the control of bacteriophage lambda PR promoter we found that transcription starting from this promoter was inhibited during the stringent, but not the relaxed, response in E. coli. We confirmed our conclusion by estimating the relative level of the PR transcript by RNA-DNA hybridization. We propose that decreased transcription from the PR promoter which serves as transcriptional activation of ori-lambda is responsible for inhibition of lambda plasmid replication during the stringent response. The results presented in this paper, combined with our recent findings (published elsewhere), indicate that the transcriptional activation of ori-lambda may be a main regulatory process controlling lambda DNA replication not only during the relaxed response but also in normal growth conditions.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 008881192

Download citation: RISBibTeXText

PMID: 8588470

Related references

Specialized nucleoprotein structures at the origin of replication of bacteriophage lambda: complexes with lambda O protein and with lambda O, lambda P, and Escherichia coli DnaB proteins. Proceedings of the National Academy of Sciences of the United States of America 82(14): 4678-4682, 1985

Transcription of the lambda dv plasmid and inhibition of lambda phages in lambda dv carrier cells of escherichia coli. Virology 41(4): 665-679, 1970

Host virus interactions in the initiation of bacteriophage lambda DNA replication. Recruitment of Escherichia coli DnaB helicase by lambda P replication protein. Journal of Biological Chemistry 265(22): 13297-13307, 1990

Initiation of bacteriophage lambda dna replication with 9 purified lambda and escherichia coli replication proteins. Journal of Cellular Biochemistry Suppl. (10 Part B): 170, 1986

Inhibition of escherichia coli dnab helicase activity by the bacteriophage lambda p replication protein. FASEB Journal 4(7): A2293, 1990

Guanosine tetraphosphate (ppGpp)-mediated inhibition of the activity of the bacteriophage lambda pR promoter in Escherichia coli. Molecular & General Genetics 257(4): 490-495, 1998

Specificity determinants for bacteriophage lambda DNA replication. III. Activation of replication in lambda ric mutants by transcription outside of ori. Journal of Molecular Biology 154(1): 65-83, 1982

Toxicity of the bacteriophage lambda cII gene product to Escherichia coli arises from inhibition of host cell DNA replication. Virology 313(2): 622-628, 2003

Explanations accounting for transduction by bacteriophage lambda in maltose negative bacteriophage lambda resistant mutants of Escherichia coli K-12. Molecular and General Genetics 159(2): 143-149, 1978

Rna polymerase bound to the p r promoter of bacteriophage lambda inhibits open complex formation at the divergently transcribed p r m promoter implications for an indirect mechanism of transcriptional activation by lambda repressor. Journal of Molecular Biology 222(3): 479-494, 1991

ClpP/ClpX-mediated degradation of the bacteriophage lambda O protein and regulation of lambda phage and lambda plasmid replication. Archives of Microbiology 174(1-2): 89-96, 2000

Regulation of lambda rex expression after infection of Escherichia coli K by lambda bacteriophage. Journal of Virology 9(3): 510-518, 1972

Initiation of lambda DNA replication reconstituted with purified lambda and Escherichia coli replication proteins. Biochimica et Biophysica Acta 951(2-3): 344-350, 1988

DNA looping induced by bacteriophage lambda O protein: implications for formation of higher order structures at the lambda origin of replication. Virology 168(2): 370-377, 1989

Initiation of the DNA replication of bacteriophage lambda in Escherichia coli K12. Journal of Molecular Biology 113(1): 1-25, 1977