Section 9
Chapter 8,894

Interaction of structurally diverse pesticides with the human MDR1 gene product P-glycoprotein

Bain, L.J.; LeBlanc, G.A.

Toxicology and Applied Pharmacology 141(1): 288-298


ISSN/ISBN: 0041-008X
PMID: 8917702
DOI: 10.1016/s0041-008x(96)80035-4
Accession: 008893033

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P-glycoprotein (P-gp) is a 170-kDa membrane-bound glycoprotein shown to efflux a wide variety of chemicals, such as chemotherapeutic agents and carcinogens. Experiments were conducted using B16/F10 murine melanoma cells transfected with the human MDR1 gene (B16/hMDR1 cells), which codes for P-gp, to determine whether this transporter may contribute to the cellular efflux of some pesticides. Thirty-eight pesticides representing several classes of compounds were evaluated for their potential to bind to P-gp, as measured by the inhibition of efflux of the P-gp substrate doxorubicin. Carbamate and pyrethroid insecticides exhibited little interaction with P-gp, while many of the organophosphorus and organochlorine pesticides significantly inhibited the efflux of doxorubicin. Pesticides that significantly inhibited the efflux of doxorubicin were then assessed for P-gp-mediated efflux. One pesticide, endosulfan, exhibited slight though significant transport mediated by P-gp. Competition experiments performed with the P-glycoprotein ligand (3H)azidopine demonstrated that the P-gp inhibitory pesticides bound to P-gp. Both lipophilicity and molecular mass were major physical/chemical determinants in dictating pesticide binding to P-gp, with optimum binding occurring with compounds having a log K-ow, value of 3.6-4.5 and a molecular weight of 391-490 Da. The transport substrate endosulfan possessed optimal binding characteristics. These results demonstrated that many pesticides are capable of binding to P-gp; however, binding does not infer transport.

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