EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Interference of the antihormone RU486 in the determination of testosterone and estradiol by enzyme-immunoassay


Interference of the antihormone RU486 in the determination of testosterone and estradiol by enzyme-immunoassay



Clinica Chimica Acta; International Journal of Clinical Chemistry 275(1): 63-69



ISSN/ISBN: 0009-8981

PMID: 9706844

DOI: 10.1016/s0009-8981(98)00077-1

The antihormone RU486 (mifepristone, 11beta-(4-dimethylaminophenyl)-17beta-hydroxy-17alpha-(prop- 1-ynyl)-estra-4,9-dien-3-one) is currently used in many endocrinological studies and in clinical practice as a contraceptive agent. The results presented here indicate that the synthetic steroid RU486 may interfere in determinations of testosterone and estradiol when using some commercial kits. Although the cross reactivity is low (0.30% for testosterone and 0.16% for estradiol), in animal experiments RU486 doses higher than 5 mg/kg may produce false positive results in the estimation of plasma testosterone (specially in castrated male or female mice) or estradiol concentrations, even in samples obtained 48 h after the administration of this antihormone.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008896213

Download citation: RISBibTeXText

Related references

Interference of serum proteins during the quantitative determination of digoxin in the blood by various immunological methods enzyme multiplied immunoassay technique enzyme linked immuno sorbent assay radio immunoassay. Therapie (London/Paris) 38(1): 117-118, 1983

Determination of the concentrations of the steroids estradiol, progesterone and testosterone in bovine sera: comparison of commercial dissociation enhanced lanthanide fluorescence immunoassay kits with conventional radio and enzyme immunoassays. Analyst 120(6): 1827-1830, 1995

The radio immunoassay of testosterone estradiol 17 beta and progesterone for sex determination from dried blood stains. Journal of Nuclear Medicine 23(5): P40, 1982

Enzyme immunoassay of 17 beta-estradiol, estrone conjugates, and testosterone in urinary and fecal samples from male and female mice. Hormone and Metabolic Research 33(11): 653-658, 2001

Development of an enzyme immunoassay for the determination of testosterone. Turk Veterinerlik ve Hayvanclk Dergisi 27(1): 45-50, 2003

Simplified methods for the determination of testosterone estradiol binding globulin capacity and percent bound of testosterone and estradiol to testosterone estradiol binding globulin. Folia Endocrinologica Japonica 56(10): 1461-1474, 1980

Interference between eplerenone and digoxin in fluorescence polarization immunoassay, microparticle enzyme immunoassay, and affinity column-mediated immunoassay. Therapeutic Drug Monitoring 32(6): 774-777, 2010

Determination of estradiol by biotin-avidin-amplified electrochemical enzyme immunoassay. Analytical Letters 40(5): 887-896, 2007

Determination of plasma 17 beta estradiol using an enzyme immunoassay kit comparison with a specific radioimmunoassay. Journal of Steroid Biochemistry 33(4 Part B): 833-836, 1989

Microtitre plate enzyme immunoassay for determination of estradiol-17-beta in bovine plasma. Journal of Reproduction and Development 43(5): 9-14, 1997

Enzyme immunoassay for specific determination of the synthetic estrogen, ethynyl estradiol, in plasma. Clinical Chemistry 27(6): 901-905, 1981

Enzyme immunoassay of testosterone, 17beta-estradiol, and progesterone in perspiration and urine of preadolescents and young adults: exceptional levels in men's axillary perspiration. Hormone and Metabolic Research 40(11): 819-826, 2008

A rapid and direct enzyme immunoassay elisa for determination of serum estradiol for in vitro fertilisation. Acta Endocrinologica Suppl 122(1): 87, 1990

Rheumatoid factor in acute viral infections: interference with determination of IgM, IgG, and IgA antibodies in an enzyme immunoassay. Journal of Infectious Diseases 142(2): 250-255, 1980

Development of an enzyme immunoassay variant for the determination of testosterone and its use for the evaluation of gonadal androgenic activity. Problemy Endokria 36(6): 44-48, 1990