Section 9
Chapter 8,899

Interleukin-12 decreases human immunodeficiency virus type 1 replication in human macrophage cultures reconstituted with autologous peripheral blood mononuclear cells

Akridge, R.E.; Reed, S.G.

Journal of Infectious Diseases 173(3): 559-564


ISSN/ISBN: 0022-1899
PMID: 8627017
DOI: 10.1093/infdis/173.3.559
Accession: 008898700

Download citation:  

In vitro interactions between interleukin (IL)-12, interferon (IFN)-gamma, and human immunodeficiency virus (HIV) type 1 infection in human macrophages were examined. Macrophages were infected with HIV-1 and cocultured with autologous monocyte-depleted peripheral blood mononuclear cells (PBMC). The addition of autologous PBMC to HIV-1-infected macrophages resulted in an expansive increase in reverse transcriptase (RT) activity; however, when both autologous PBMC and IL-12 were added, RT activity decreased (75%-90%) and high levels of IFN-gamma (9-16 ng/mL) were detected. The addition of anti-IFN-gamma antibodies blocked the IL-12-induced decrease in RT activity. Surprisingly, exogenous IL-12 added to HIV-infected macrophage cultures without autologous lymphocytes resulted in a 50%-60% reduction in RT activity and no detectable increase in IFN-gamma. The addition of anti-IFN-gamma did not inhibit this IL-12-mediated effect. These results suggest that IL-12 is capable of indirectly down-regulating HIV proliferation in macrophage cultures reconstituted with autologous PBMC and of directly suppressing HIV replication in purified macrophage cultures.

PDF emailed within 0-6 h: $19.90