EurekaMag.com logo
+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Interspecies protoplast fusion in Larix: Comparison of electric and chemical methods



Interspecies protoplast fusion in Larix: Comparison of electric and chemical methods



In Vitro Cellular & Developmental Biology Plant 34(3): 212-217, July-Sept



Larix was chosen for the study on interspecies protoplast fusion due to its ability to regenerate plants from protoplasts derived from embryogenic cultures. L. laricina line L2 was used in fusion experiments with either L. X eurolepis line L6 or L. X leptoeuropaea line L5. A method of unambiguous labeling of parental protoplasts prior to fusion was developed using vital fluorescent dyes. Of a number of dyes tested, only rhodamine B hexyl ester chloride (R6) and 3,3'-dihexyloxcarbocyanine iodide (DiOC6) stained the protoplasts in a consistent and uniform fashion. The fusion of mixed parental protoplasts that were internally labeled was carried out either in the presence of a 20% polyethylene glycol (PEG) solution or in an electric field. The progress of fusion was readily observed, taking only minutes under the experimental conditions. The fusion products could be identified by dual fluorescence several h after the onset of fusion. Heterofusion frequencies of approximately 18% and 6% in the presence of PEG and an electric field, respectively, were attained. Postfusion cultures between L. X laricina protoplasts and protoplasts of L. X leptoeuropaea gave rise, to cell colonies and between L. laricina and L. X eurolepis, to mature somatic embryos.

(PDF emailed within 0-6 h: $19.90)

Accession: 008901996

Download citation: RISBibTeXText

DOI: 10.2307/20064985



Related references

Protoplast fusion of lactobacillus acidophilus and streptococcus lactis via electric field or chemical induction. Journal of General & Applied Microbiology 33(3): 287-294, 1987

Protoplast fusion and interspecies hybridization in Penicillium. Mycology series(6): 259-277, 1985

Interspecies hybridization after protoplast fusion in Aspergillus. Mycology series(6): 241-257, 1985

Virus transmission through interspecies protoplast fusion in aspergillus. Transactions of the British Mycological Society 89(1): 73-82, 1987

Fusion of Bacillus subtilis and Bacillus licheniformis protoplasts. Interspecies recombination resulting from protoplast fusion. Antibiotiki i Meditsinskaia Biotekhnologiia 30(9): 643-649, 1985

Fusion of protoplasts of bacillus subtilis and bacillus licheniformis interspecies recombination resulting from protoplast fusion. Antibiotiki i Meditsinskaya Biotekhnologiya 30(9): 643-649, 1985

Intra- and interspecies virus transfer in Aspergilli via protoplast fusion. Fungal Genetics and Biology 25(3): 171-180, 1999

Effects of incompatibility on protoplast fusion between intraspecies and interspecies in basidiomycete pleurotus spp. Korean Journal of Mycology 17(3): 137-144, 1989

Protoplast fusion in banana (Musa spp.): comparison of chemical (PEG: polyethylene glycol) and electrical procedure. Plant Cell, Tissue and Organ Culture 83(2): 145-151, 2005

Microbiological implications of electric field effects. III. Stimulation of yeast protoplast fusion by electric field pulses. Zeitschrift für Allgemeine Mikrobiologie 21(7): 555-562, 1981

Microbial implications of electric field effects. III. Stimulation of yeast protoplast fusion by electric field pulses. Zeitschrift fur allgemeine Mikrobiologie1(7): 555-562, 1981

Basic studies on protoplast fusion in woody plants I. Investigation on the methods of fusion and selection. Technical Note, Oji Institute for Forest Tree Improvement, Japan (240): i + 3, 1985

Isolation of interspecies hybrids of penicillium citrinum and penicillium cyaneofulvum following protoplast fusion. FEMS Microbiology Letters 4(2): 87-90, 1978

Interspecies recombination by protoplast fusion in streptomyces lincolnensis and streptomyces venezuelae. Zhongguo Kanfshengsu Zazhi 14(3): 192-196, 1989