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Intraspecies hybridization of yeasts Candida tropicalis and Candida utilis



Intraspecies hybridization of yeasts Candida tropicalis and Candida utilis



Mikrobiolohichnyi Zhurnal 58(4): 43-47



Hybrid prototrophs with occurrence frequency 3.4 cntdot 10-2 - 1 cntdot 10-5, splitting of which indicates haploidy of initial parent strains have been obtained by means of the method of fusion of protoplasts of single and double auxotrophic mutants of yeast Candida tropicalis. Histidine-dependent auxotrophs prevail among the forms splitted-out by prototrophic hybrids, and this evidences for the prevalence of one of parental genoms under formation of the hybrid caryotype from mutants with similar demand. Clones with one, two and three demands in the growth factors appear under splitting of hybrids formed as a result of fusion of double auxotrophs and this to confirm participation of the both parents in formation of the nucleus of hybrid strain. Hybridization of C. utilis by the sex crossing of cells of auxotrophic strains of yeast of the opposite type of pairing (A and B) and further splitting of hybrids indicate to haploidy of initial forms. Hybrids obtained are distinguished by various character of inclusion of parent markers to their genom: the mixed genotypes are observed parallel with prevalence of one of the initial genotypes.

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