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Isolation and characterization of temperature sensitive mutants of Bombyx mori nuclear polyhedrosis virus and their multiplication in a cell line and larvae of Bombyx mori



Isolation and characterization of temperature sensitive mutants of Bombyx mori nuclear polyhedrosis virus and their multiplication in a cell line and larvae of Bombyx mori



Journal of Sericultural Science of Japan 64(3): 230-236



Temperature sensitive (ts) mutants (No. 128 and No. 217) of Bombyx mori nuclear polyhedrosis virus (BmNPV) were isolated by the plaque formation method from BmN-4 cells after the inoculation of BmNPV which had been treated with 5-bromo-2'-deoxyuridine. These 2 ts mutants were characterized, together with a previously established one (No. 20). At 25 degree C the yield of the progeny viruses of the 3 ts mutants did not differ from the wild type of BmNPV. At 33 degree C, however, the rates of viral replication of the mutants No. 20 and No. 128 were markedly inhibited. Multiplication of the mutant No. 217 was inhibited slightly and the polyhedron formation and viral replication were decreased. Also observed was abnormal virus-envelope formation in the fat body cells. In the case where culture temperature was shifted from 25 to 33 degree C postinfection of the ts mutants, viral replication was inhibited both in the cell line and in larvae. When the temperature was shifted back to 25 degree C, the ts mutants again replicated normally in the cell line and in larvae. These results indicated that the inhibition of virus replication was not caused by the defect in the viral absorption pathway. Coinfection of the ts mutant No. 128 and others exhibited a complementation phenomenon and polyhedra formed were increased at 33 degree C.

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Accession: 008925100

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