+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Isolation and characterization of transformed human T-cell lines infected by Epstein-Barr virus



Isolation and characterization of transformed human T-cell lines infected by Epstein-Barr virus



Blood 89(12): 4521-4530



Epstein-Barr virus (EBV) is a human lymphotropic virus whose main targets have traditionally been described as B lymphocytes and epithelial cells. Here we report the isolation and characterization of largely monoclonal transformed human T-cell lines infected by EBV. The transformed T cells expressed CD2, CD3, and either CD4 or CD8 surface molecules and more generally displayed the phenotype of naive T cells with a complete and clonal rearrangement of the T-cell receptor. None of the cell lines expressed B cells, natural killer, or myeloid antigens or had immunoglobulins genes rearrangement. They grew in the absence of growth factor, however, they all secreted interleukin-2 after mitogenic activation. Polymerase chain reaction (PCR) analysis showed the presence of EBV DNA in all these cell lines. Moreover, Southern blot analysis of one of these cell lines shows the presence of circular episomic EBV DNA, and by Northern blot or reverse transcriptase-PCR analysis, only the expression of Epstein-Barr nuclear antigen-1 (EBNA-1) and latent membrane protein-1 (LMP-1) genes was detected. Finally, the complete transformed phenotype of this T-cell line was shown by its injection into nude or recombination activating gene 2 (RAG2)-deficient mice that led to the formation of solid tumors.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 008925368

Download citation: RISBibTeXText

PMID: 9192776


Related references

Purification of the Epstein-Barr virus-determined nuclear antigen from Epstein-Barr virus-transformed human lymphoid cell lines. Journal of Virology 27(3): 604-611, 1978

Establishment of epstein barr virus nuclear antigen expressing cell lines by infection of epstein barr virus genome negative human lymphoma cells with different epstein barr virus strains. International Journal Of Cancer: 161-166, 1976

Chromosomal localization of the epstein barr virus genome in bloom's syndrome b lymphoblastoid cell lines transformed with epstein barr virus. Chromosoma 93(2): 157-164, 1985

Isolation and characterization of Epstein-Barr virus-infected clones of a human T-lymphoblastoid cell line. Nihon Rinsho. Japanese Journal of Clinical Medicine 55(2): 416-423, 1997

Relationship between the amounts of epstein barr virus dna and epstein barr virus nuclear antigen per cell clonability and tumorigenicity in 2 epstein barr virus negative lymphoma lines and their epstein barr virus converted sublines. International Journal of Cancer 31(2): 163-170, 1983

Establishment and characterization of six human gastric carcinoma cell lines, including one naturally infected with Epstein-Barr virus. Cellular Oncology 35(2): 127-136, 2012

Characterization of stable Epstein-Barr (EB) virus transformed cell lines and mouse-human hybridomas producing a large quantity of anti-tetanus toxoid (TT) monoclonal antibody. Behring Institute Mitteilungen 1985(78): 139-147, 1985

Epstein-Barr virus (EBV) expression in transformed human lymphoblastoid cell lines from different sources. Bibliotheca Haematologica 1975(43): 406-408, 1975

Sensitivity of the Epstein-Barr virus transformed human lymphoid cell lines to interferon. Journal of General Virology 28(2): 207-217, 1975

Use of cyclosporin A in establishing Epstein-Barr virus-transformed human lymphoblastoid cell lines. In Vitro 20(11): 856-858, 1984

Use of Cyclosporin a in Establishing Epstein-Barr Virus-Transformed Human Lymphoblastoid Cell Lines. In Vitro 20(11): 856-858, 1984

Production of human monoclonal antibodies by Epstein-Barr virus-transformed cell lines. Harefuah 107(7-8): 167-168, 1984

Heterotransplantation of two human lymphoid cell lines transformed in vitro by Epstein-Barr virus. Journal of the National Cancer Institute 47(4): 771-780, 1971

Characterization of epstein-barr virus-infected mantle cell lymphoma lines. Acta Medica Okayama 54(5): 193-200, 2000

Early human igh gene assembly in epstein barr virus transformed fetal b cell lines. Journal of Experimental Medicine 169(4): 1391-1404, 1989