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Isolation of ampicillin sensitive Aeromonas from aquatic foods using xylose-lysine-deoxycholate agar and ampicillin-dextrin agar



Isolation of ampicillin sensitive Aeromonas from aquatic foods using xylose-lysine-deoxycholate agar and ampicillin-dextrin agar



Journal of Food Science & Technology 30(5): 359-361



In the present study, 96 fresh waterfishes, 37 marine fishes, 13 fresh water prawns, 13 marine shrimps and 26 molluscs have been screened for Aeromonas sp. by plating on xylose-lysine-desoxycholate agar (XLDA) and ampicillin-dextrin agar (ADA), after enrichment in alkaline broth. A total of 13 Aeromonas strains were isolated with the use of XLDA; 8 from fresh waterfishes, 4 from molluscs and one from marine shrimp. Among Aeromonas isolates, 11 were A. sobria, one each was A. salmonicida subsp. salmonicida and A. salmonicida subsp. masoucida. With the use of ADA, only 7 samples were positive for Aeromonas, while the undetected isolates were the ampicillin-sensitive strains. Among the isolates, 5 were sensitive t all the 10 antibiotics tested, while the sensitivity to other antibiotics varied widely. None of the isolates was resistant to nalidixic acid, oxytetracycline, chloramphenicol, norflox and doxycycline.

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