+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Kinetics of cytokine mRNA expression in the central nervous system following lethal and nonlethal coronavirus-induced acute encephalomyelitis



Kinetics of cytokine mRNA expression in the central nervous system following lethal and nonlethal coronavirus-induced acute encephalomyelitis



Virology 233(2): 260-270



The potential role(s) of cytokines in the reduction of infectious virus and persistent viral infection in the central nervous system was examined by determining the kinetics of cytokine mRNA expression following infection with the neurotropic JHM strain of mouse hepatitis virus. Mice were infected with an antibody escape variant which produces a nonlethal encephalomyelitis and compared to a clonal virus population which produces a fulminant fatal encephalomyelitis. Infection with both viruses induced the accumulation of mRNAs associated with Th1- and Th2-type cytokines, including IFN-gamma, IL-4, and IL-10. Peak mRNA accumulations were coincident with the clearance of virus and there was no obvious differences between lethally and nonlethally infected mice. TNF-alpha mRNA was induced more rapidly in lethally infected mice compared to mice undergoing a nonfatal encephalomyelitis. Rapid transient increases in the mRNAs encoding IL-12, iNOS, IL-1alpha, IL-1beta, and IL-6 occurred following infection. Nonlethal infections were associated with increased IL-12, IL-1beta, and earlier expression of IL-6, while lethal infections were associated with increased iNOS and IL-1alpha mRNA. These data suggest a rapid but differential response within the central nervous system cells to infection by different JHMV variants. However, neither the accumulation nor kinetics of induction provide evidence to distinguish lethal infections from nonlethal infections leading to a persistent infection. Accumulation of both Th1 and Th2 cytokines in the central nervous system of JHMV-infected mice is consistent with the participation of both cytokines and cell immune effectors during resolution of acute viral-induced encephalomyelitis.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008936559

Download citation: RISBibTeXText

PMID: 9217050

DOI: 10.1006/viro.1997.8613


Related references

Expression of interleukin mRNA in lymphocytes from the central nervous system of rats with coronavirus JHM induced demyelinating encephalomyelitis. Immunobiology 189(1-2): 215, 1993

Analysis of cytokine mRNA expression in the central nervous system of mice with experimental autoimmune encephalomyelitis reveals that IL-10 mRNA expression correlates with recovery. Journal of Immunology 149(7): 2496-2505, 1992

Central nervous system cytokine mRNA expression following Theiler's murine encephalomyelitis virus infection. Journal of Neuroimmunology 76(1-2): 213-223, 1997

Dynamics of cellular cytokine mRNA expression in the central nervous system during experimental autoimmune encephalomyelitis in Lewis rats. Journal of Neuroimmunology 54(1-2): 187, 1994

Estrogen inhibits cytokine, chemokine, and chemokine receptor mRNA expression in the central nervous system of female mice with experimental autoimmune encephalomyelitis. FASEB Journal 15(5): A1032, 2001

Detection of coronavirus in the central nervous system of a child with acute disseminated encephalomyelitis. Pediatrics 113(1 Pt 1): E73-E76, 2004

17 beta-estradiol inhibits cytokine, chemokine, and chemokine receptor mRNA expression in the central nervous system of female mice with experimental autoimmune encephalomyelitis. Journal of Neuroscience Research 65(6): 529-542, 2001

Competitive PCR quantification of pro- and anti-inflammatory cytokine mRNA in the central nervous system during autoimmune encephalomyelitis. Journal Of Neuroimmunology. 73(1-2): 206, 1997

STAT mRNA kinetics in the central nervous system during autoimmune encephalomyelitis in lewis rats. Korean Journal of Veterinary Research 44(2): 163-169, 2004

Characterization of CD4+T-cells infiltrating the central nervous system of rats with coronavirus JHM-induced demyelinating encephalomyelitis. Immunobiology 189(1-2): 221, 1993

Phenotypic and functional characterization of CD8+ T lymphocytes from the central nervous system of rats with coronavirus JHM induced demyelinating encephalomyelitis. Journal of Neurovirology 1(5-6): 340-348, 1995

Cytokine gene expression in the central nervous system of rats with experimental autoimmune encephalomyelitis eae. FASEB Journal 5(6): A1780, 1991

Diminished cytokine and chemokine expression in the central nervous system of GMF-deficient mice with experimental autoimmune encephalomyelitis. Brain Research 1144: 239-247, 2007

Cytokine production in the central nervous system of Lewis rats with experimental autoimmune encephalomyelitis: dynamics of mRNA expression for interleukin-10, interleukin-12, cytolysin, tumor necrosis factor alpha and tumor necrosis factor beta. Journal of Neuroimmunology 61(2): 205-212, 1995

The critical role of antigen-presentation-induced cytokine crosstalk in the central nervous system in multiple sclerosis and experimental autoimmune encephalomyelitis. Journal of Interferon and Cytokine Research 31(10): 753-768, 2011