+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Localization of xyloglucan in the macromolecular complex composed of xyloglucan and cellulose in pea stems



Localization of xyloglucan in the macromolecular complex composed of xyloglucan and cellulose in pea stems



Plant & Cell Physiology 35(3): 439-444



A macromolecular complex composed of xyloglucan and cellulose was isolated from elongating regions of stems of etiolated pea (Pisum sativum L. var Alaska) seedlings and binding of a xyloglucan-specific antibody was examined after treatment of the complex with endo-1,4-beta-glucanase or 24% KOH. The antibody bound to the complex but the extent of binding was reduced after treatment of the complex with endo-1,4-beta-glucanase and was hardly detectable after treatment with 24% KOH. The molecular weight of the xyloglucan that remained (5%) in the beta-glucanase-treated complexes was less than 9,200. Pea xyloglucan was allowed to bind to enzyme- and alkali-treated complexes to generally reconstituted complexes. The amount of the antibody that bound to each type of reconstituted complex was similar but was much lower than that bound to the native complex. Immunogold labeling indicated that most of the antigen was widely distributed between microfibrils in the native complex, whereas the antigen appeared to be confined to the microfibrils in the reconstituted complexes. These findings suggest that a part of each xyloglucan molecule is strongly associated with cellulose microfibrils while the rest is free of the microfibrils in the native complex.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 008967321

Download citation: RISBibTeXText


Related references

Xyloglucan and xyloglucan endo-transglycosylases (XET): Tools for ex vivo cellulose surface modification. Biocatalysis and Biotransformation 24(1-2): 107-120, 2006

Xyloglucan-cellulose interaction depends on the sidechains and molecular weight of xyloglucan. Plant Physiology and Biochemistry 42(5): 389-394, 2004

Xyloglucan oligosaccharides with at least two alpha-D-xylose residues act as acceptor substrates for xyloglucan endotransglycosylase and promote the depolymerisation of xyloglucan. Physiologia Plantarum 88(1): 105-112, 1993

Xyloglucan oligosaccharides with at least two a- D-xylose residues act as acceptor substrates for xyloglucan endotransglycosylase and promote the depolymerisation of xyloglucan. Physiologia Plantarum 88: 5-12, 1993

Biosynthesis of Xyloglucan in Suspension-Cultured Soybean Cells. An Assay Method for Xyloglucan Xylosyltransferase and Attempted Synthesis of Xyloglucan from Udp-D-Xylose. The Journal of Biochemistry 89(1): 325-328, 1981

Biosynthesis of xyloglucan in suspension-cultured soybean cells. An assay method for xyloglucan xylosyltransferase and attempted synthesis of xyloglucan from UDP-D-xylose. Journal of Biochemistry 89(1): 325-328, 1981

Construction and restructuring of the cellulose-xyloglucan framework in the apoplast as mediated by the xyloglucan-related protein family-a hypothetical scheme. Journal of Plant Research 111(1101): 159-166, 1998

Pea Xyloglucan and Cellulose: Vi. Xyloglucan-Cellulose Interactions in Vitro and in Vivo. Plant Physiology 83(2): 384-389, 1987

Pea Xyloglucan and Cellulose: V. Xyloglucan-Cellulose Interactions in vitro and In vivo. Plant Physiology 83(2): 384-389, 1987

Pea xyloglucan and cellulose v. xyloglucan cellulose interactions in vitro and in vivo. Plant Physiology 83(2): 384-389, 1987

A Lytic Polysaccharide Monooxygenase with Broad Xyloglucan Specificity from the Brown-Rot Fungus Gloeophyllum trabeum and Its Action on Cellulose-Xyloglucan Complexes. Applied and Environmental Microbiology 82(22): 6557-6572, 2016

Pea xyloglucan and cellulose : I. Macromolecular organization. Plant Physiology 75(3): 596-604, 1984

Macromolecular organization of xyloglucan and cellulose in pea epicotyls. Structure function and biosynthesis of plant cell walls: proceedings Seventh Annual Symposium in Botany January 12-14-1984 University of California Riverside edited by WM Dugger and Salomon Bartnicki Garcia: 434, 1984

Macromolecular complexes of xyloglucan and cellulose obtained by annealing. Plant & Cell Physiology 35(2): 219-223, 1994

Endo-xyloglucan transferase, a novel class of glycosyltransferase that catalyzes transfer of a segment of xyloglucan molecule to another xyloglucan molecule. Journal of Biological Chemistry 267(29): 21058-21064, 1992