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Low-level oxidative stress causes cell-cycle specific arrest in cultured cells

Biochemical and Biophysical Research Communications 210(1): 189-196

Low-level oxidative stress causes cell-cycle specific arrest in cultured cells

Oxidative stress is involved in a multitude of pathological conditions. In the present study, cell cycle arrest was demonstrated in monolayer cultures of Chinese hamster ovary cells subjected to low-level oxidative stress induced by the addition of hydrogen peroxide. Fluorescence-activated cell sorting analysis characterized this arrest as occurring in both the G-1 and G-2/M phases of the cell cycle. Upon exposure to oxidative stress, cells in G-1 arrest in G-1, while S-phase cells complete DNA synthesis and subsequently arrest in G-2/M. Nitroxide spin labels with differing permeabilities are able to protect the cells, which suggests that both the G-1 and the G-2M arrest involve oxidation of targets located at the cell surface.

Accession: 008976507

PMID: 7741740

DOI: 10.1006/bbrc.1995.1645

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