EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Lysosomal enzyme trafficking between phagosomes, endosomes, and lysosomes in J774 macrophages. Enrichment of cathepsin H in early endosomes


Lysosomal enzyme trafficking between phagosomes, endosomes, and lysosomes in J774 macrophages. Enrichment of cathepsin H in early endosomes



Journal of Biological Chemistry 273(16): 9842-9851



ISSN/ISBN: 0021-9258

PMID: 9545324

DOI: 10.1074/jbc.273.16.9842

In this study we take advantage of recently developed methods using J774 macrophages to prepare enriched fractions of early endosomes, late endosomes, dense lysosomes, as well as phagosomes of different ages enclosing 1-micron latex beads to investigate the steady state distribution and trafficking of lysosomal enzyme activity between these organelles. At steady state these cells appear to possess four different cellular structures, in addition to phagolysosomes, where acid hydrolases were concentrated. The first site of hydrolase concentration was the early endosomes, which contained the bulk of the cellular cathepsin H. This enzyme was acquired by phagosomes significantly faster than the other hydrolases tested. The second distinct site of lysosomal enzyme concentration was the late endosomes which contain the bulk of cathepsin S. The third and fourth large pools of hydrolases were found in two functionally distinct types of dense lysosomes, only one of which was found to be secreted in the presence of chloroquine or bafilomycin. Among this secreted pool was soluble furin, generally considered only as a membrane-bound trans-Golgi network resident protein. Thus, the organelles usually referred to as "lysosomes" in fact encompass a growing family of highly dynamic but functionally distinct endocytic organelles.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 008980661

Download citation: RISBibTeXText

Related references

Inhibitory effect of Toll-like receptor 4 on fusion between phagosomes and endosomes/lysosomes in macrophages. Journal of Immunology 172(4): 2039-2047, 2004

Alterations in lysosomal enzyme and membrane protein distribution in the End6 mutant support recycling of hydrolases from dense lysosomes to early endosomes. Molecular Biology of the Cell 5(Suppl ): 77A, 1994

Isolation and characterization of early endosomes, late endosomes and terminal lysosomes: their role in protein degradation. Journal of Cell Science 109: 2905-2914, 1996

Early endosomes, late endosomes, and lysosomes display distinct partitioning strategies of inheritance with similarities to Golgi-derived membranes. European Journal of Cell Biology 81(3): 117-124, 2002

The porcine reproductive and respiratory syndrome virus requires trafficking through CD163-positive early endosomes, but not late endosomes, for productive infection. Archives of Virology 154(12): 1939-1943, 2009

Involvement of early endosomes in the trafficking of the CI-mannose 6-phosphate receptor and lysosomal proteins. Molecular Biology of the Cell 8(Suppl ): 423A, 1997

Ubiquitylation of the gap junction protein connexin-43 signals its trafficking from early endosomes to lysosomes in a process mediated by Hrs and Tsg101. Journal of Cell Science 122(Pt 21): 3883-3893, 2009

Recycling of lysosomal hydrolases and lysosomal membrane glycoproteins from lysosomes to endosomes in the end6 mutant of CHO cells. Molecular Biology of the Cell 6(Suppl ): 215A, 1995

Transport from Late Endosomes to Lysosomes, but Not Sorting of Integral Membrane Proteins in Endosomes, Depends on the Vacuolar Proton Pump. Journal of Cell Biology 130(4): 821-834, 1995

Phagosomes fuse with late endosomes and/or lysosomes by extension of membrane protrusions along microtubules: Role of Rab7 and RILP. Molecular and Cellular Biology 23(18): 6494-6506, 2003

Lysosomal acid phosphatase is transported via endosomes to lysosomes. Biochemical and Biophysical Research Communications 166(3): 1176-1182, 1990

The lysosomal membrane glycoprotein lgp107 is transported via endosomes to lysosomes. Cell Structure and Function 14(6): 930, 1989

Methods of analysis of the membrane trafficking pathway from recycling endosomes to lysosomes. Methods in Enzymology 534: 195-206, 2014

Endosomes, lysosomes, and the role of endosomal and lysosomal biogenesis in cancer development. Molecular Biology Reports 47(12): 9801-9810, 2020

Rab5 regulates the kiss and run fusion between phagosomes and endosomes and the acquisition of phagosome leishmanicidal properties in RAW 264.7 macrophages. Journal of Cell Science 113 Pt 19: 3531-3541, 2000