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Malic enzyme: Its purification and characterization from Mucor circinelloides and occurrence in other oleaginous fungi



Malic enzyme: Its purification and characterization from Mucor circinelloides and occurrence in other oleaginous fungi



World Journal of Microbiology and Biotechnology 13(1): 7-9



Malic enzyme was purified 43-fold from Mucor circinelloides. The enzyme was dependent on Mg-2+ or Mn-2+ for activity, was not active with D-malate and had a pH optimum at 7.8. The apparent K-m values for malate and NADP+ were 488 mu-M and 41 mu-M respectively. The M-r of the native enzyme was 160 kDa. Five metabolic analogues of malate: oxaloacetate, tartronic acid, 1-methylenecyclopropane trans-2,3-dicarboxylic acid, malonic acid and glutaric acid, were found to inhibit malic enzyme activity at 10 mM. Four oleaginous fungi, Mucor circinelloides, Mortierella alpina, Mortierella elongata and Pythium ultimum, were also examined, all possessed a soluble malic enzyme, two also possessed a microsomal malic enzyme.

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