Manganese-dependent Dopa/tyrosine sulfation in HepG2 human hepatoma cells: Novel Dopa/tyrosine sulfotransferase activities associated with the human monoamine-form phenol sulfotransferase
Sakakibara, Y.; Katafuchi, J.; Takami, Y.; Nakayama, T.; Suiko, M.; Nakajima, H.; Liu, M.C.
Biochimica et Biophysica Acta 1355(2): 102-106
Human monoamine (M)-form phenol sulfotransferase (PST) was PCR-cloned and transiently expressed in COS-7 cells. The recombinant enzyme was demonstrated to display not only the previously reported sulfotransferase activity toward dopamine, but also novel manganese-dependent Dopa/tyrosine sulfotransferase activities. These results imply a new functional role of the human M-form PST in the homeostatic regulation of Dopa and tyrosine.