EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Measurement of S phase duration in human epidermis using cyclin immunostaining and tritiated-thymidine pulse labelling


Archives of Dermatological Research 284(4): 238-241
Measurement of S phase duration in human epidermis using cyclin immunostaining and tritiated-thymidine pulse labelling
Cyclin/PCNA (proliferating cell nuclear antigen) is a nuclear protein strongly associated with DNA replication sites. Under methanol fixation, cyclin immunostaining might serve as a specific marker for S-phase cells. Associated with single labelling with 3H-thymidine, this immunodetection represents an easy method for measuring S-phase duration. Its applications to six samples or normal human epidermis allowed us to measure an S phase of 9.7 +- 0.3 h, a results in fair agreement with a previous estimate of 10.2 h obtained from a classical double-labelling technique with two different concentrations of 3H-thymidine.


Accession: 008998310



Related references

Measurement of S phase duration in human epidermis using cyclin immunostaining and 3H-thymidine pulse labelling. Archives of Dermatological Research 284(4): 238-241, 1992

S phase duration measurement by combined pcna cyclin immunostaining and radioautography after a single pulse labelling with tritiated thymidine. Cell Biology International Reports 14(9): 765-774, 1990

S phase duration measurement by combined PCNA/cyclin immunostaining and radioautography after a single pulse-labelling with 3H-thymidine. Cell Biology International Reports 14(9): 765-774, 1990

Cyclin pnca immunostaining as an alternative to tritiated thymidine pulse labelling for marking s phase cells in paraffin sections from animal and human tissues. Cell & Tissue Kinetics 22(5): 383-392, 1989

Cyclin/PCNA immunostaining as an alternative to tritiated thymidine pulse labelling for marking S phase cells in paraffin sections from animal and human tissues. Cell and Tissue Kinetics 22(5): 383-392, 1989

Measurement of the S-phase duration in normal and abnormal human endometrium by in vitro double labelling with bromodeoxyuridine and tritiated thymidine. Journal of Pathology 157(2): 109-115, 1989

Estimation of S phase duration in goat epidermis by an in vivo intradermal double labelling technique using bromodeoxyuridine and tritiated thymidine. Research in veterinary science 52(1): 5-9, 1992

Ki-67 immunostaining of normal human epidermis: comparison with 3H-thymidine labelling and PCNA immunostaining. Dermatology 197(2): 123-126, 1998

Snap labelling of s phase cells with bromodeoxyuridine in normal hyperplastic and malignant human endometrium a comparison with tritiated thymidine labelling. Journal of Pathology 152(3): 191A, 1987

Estimation of S phase duration in goat epidermis by an in vivo intradermal double labeling technique using bromodeoxyuridine and tritiated thymidine. Research in Veterinary Science 52(1): 5-9, 1992