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Measurement of S phase duration in human epidermis using cyclin immunostaining and tritiated-thymidine pulse labelling



Measurement of S phase duration in human epidermis using cyclin immunostaining and tritiated-thymidine pulse labelling



Archives of Dermatological Research 284(4): 238-241



Cyclin/PCNA (proliferating cell nuclear antigen) is a nuclear protein strongly associated with DNA replication sites. Under methanol fixation, cyclin immunostaining might serve as a specific marker for S-phase cells. Associated with single labelling with 3H-thymidine, this immunodetection represents an easy method for measuring S-phase duration. Its applications to six samples or normal human epidermis allowed us to measure an S phase of 9.7 +- 0.3 h, a results in fair agreement with a previous estimate of 10.2 h obtained from a classical double-labelling technique with two different concentrations of 3H-thymidine.

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Accession: 008998310

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