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Method for generation of human B lymphoblastoid cell lines using Epstein-Barr virus

Method for generation of human B lymphoblastoid cell lines using Epstein-Barr virus

Methods in Cell Science 17(2): 67-74

ISSN/ISBN: 1381-5741

DOI: 10.1007/bf00986653

A detailed methodology is described for the successful immortalization of B lymphocytes to give lymphoblastoid cell lines using Epstein-Barr (EB) virus. A virus stock is prepared from B95-8 or Akata cell line, which spontaneously release high titers of virus, and the immortalizing titer of the preparation determined. B lymphocytes from fresh or cryopreserved samples are separated from the whole cell population, infected with EB virus and cultured for 4 weeks for the establishment of a lymphoblastoid cell line. The presence of EB virus encoded proteins in the immortalized cell line can be detected by indirect immunofluorescent staining.

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Accession: 009016518

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