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The cranial nerves of Wallago attu
A dissection of the Weberian ossicles
A study on urpad beel in Goalpara District, Assam
Cresyl violet staining in exfoliative gynecologic cytology
Treatment of fibroids: the use of beets (Beta vulgaris) and molasses (Saccharum officinarum) as an herbal therapy by Dominican healers in New York City
The Shahada movement: a peasant movement in Maharashtra (India) - its development and its perspectives
Economic importance of Pteridophytes
Stassanisation of Milk
Anatomy of Mystus seenghala IV Nervous system
The evolution of the coelom and metameric segmentation
1. The Integrated Project Planning and Management Cycle (IPPMC)
Cytogenetic analysis of ZZWW triploid silkworm (Bombyx mori)
Contribution to the study of vitellogenesis in birds Physiological phases and the role of folliculin in vitellogenesis
Some Biological Applications of Organometallic Compounds
Quaternary geology and geomorphology in parts of Kohima, Wokha, Mokokchung and Mon districts, Nagaland
A dissection of the Weberian ossicles
A study on urpad beel in Goalpara District, Assam
Cresyl violet staining in exfoliative gynecologic cytology
Treatment of fibroids: the use of beets (Beta vulgaris) and molasses (Saccharum officinarum) as an herbal therapy by Dominican healers in New York City
The Shahada movement: a peasant movement in Maharashtra (India) - its development and its perspectives
Economic importance of Pteridophytes
Stassanisation of Milk
Anatomy of Mystus seenghala IV Nervous system
The evolution of the coelom and metameric segmentation
1. The Integrated Project Planning and Management Cycle (IPPMC)
Cytogenetic analysis of ZZWW triploid silkworm (Bombyx mori)
Contribution to the study of vitellogenesis in birds Physiological phases and the role of folliculin in vitellogenesis
Some Biological Applications of Organometallic Compounds
Quaternary geology and geomorphology in parts of Kohima, Wokha, Mokokchung and Mon districts, Nagaland
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Identification of compounds with anti-West Nile Virus activity
Geological age of the Ptilophyllum flora; a critical reassessment
Nira (Co 86032) - a new variety of sugarcane for Maharashtra
Occurrence of Eutrichophilus mexicanus (Rudow, 1866) and Eutrichophilus lobatus (Ewing, 1936) (Phthiraptera: Trichodectidae) on Sphiggurus villosus (Cuvier, 1825) (Rodentia: Erethizontidae) in Rio de
An assessment of water quality of river ganga at garhmukteshwar ghaziabad uttar pradesh india
The identity of the lipstick mold of cultivated mushrooms agaricus bisporus
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
The composition of pampas-grass (Cortaderia argentea.)
Studies on feeding of tapioca thippi (tapioca or cassava starch waste) to swine
Effects of drum rolling on yield of summer groundnut
Integrated farming system for gardenland conditions of Coimbatore district - an over view
Influence of a decision analysis model on selection of drug therapy
Comparative performance of West Coast Tall and Lakshadweep Ordinary cultivars of coconut
Evaluation of WCT coconut and Komadan coconut
Cellars as hibernation sites for bats
Donkioporia expansa - A lesser known wood destroyer in buildings
The Bangla adaptation of Mini-Mental State Examination (BAMSE): An instrument to assess cognitive function in illiterate and literate individuals
Enterobacter amnigenus. An unusual human pathogen
'Rajeshwari' - a high-yielding white seeded variety of sesame for Andhra Pradesh
Gymnosperms of Sikkim, India
The great Coalinga cricket invasion
Psoriasis: correlation between severity index (PASI) and quality of life index (DLQI) in patients assessed before and after systemic treatment
Real-time PCR assays for the specific detection of monkeypox virus West African and Congo Basin strain DNA
Classification of fermented foods: worldwide review of household fermentation techniques
The biology of human starvation: some new insights
Geological age of the Ptilophyllum flora; a critical reassessment
Nira (Co 86032) - a new variety of sugarcane for Maharashtra
Occurrence of Eutrichophilus mexicanus (Rudow, 1866) and Eutrichophilus lobatus (Ewing, 1936) (Phthiraptera: Trichodectidae) on Sphiggurus villosus (Cuvier, 1825) (Rodentia: Erethizontidae) in Rio de
An assessment of water quality of river ganga at garhmukteshwar ghaziabad uttar pradesh india
The identity of the lipstick mold of cultivated mushrooms agaricus bisporus
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
The composition of pampas-grass (Cortaderia argentea.)
Studies on feeding of tapioca thippi (tapioca or cassava starch waste) to swine
Effects of drum rolling on yield of summer groundnut
Integrated farming system for gardenland conditions of Coimbatore district - an over view
Influence of a decision analysis model on selection of drug therapy
Comparative performance of West Coast Tall and Lakshadweep Ordinary cultivars of coconut
Evaluation of WCT coconut and Komadan coconut
Cellars as hibernation sites for bats
Donkioporia expansa - A lesser known wood destroyer in buildings
The Bangla adaptation of Mini-Mental State Examination (BAMSE): An instrument to assess cognitive function in illiterate and literate individuals
Enterobacter amnigenus. An unusual human pathogen
'Rajeshwari' - a high-yielding white seeded variety of sesame for Andhra Pradesh
Gymnosperms of Sikkim, India
The great Coalinga cricket invasion
Psoriasis: correlation between severity index (PASI) and quality of life index (DLQI) in patients assessed before and after systemic treatment
Real-time PCR assays for the specific detection of monkeypox virus West African and Congo Basin strain DNA
Classification of fermented foods: worldwide review of household fermentation techniques
The biology of human starvation: some new insights
Method for generation of human B lymphoblastoid cell lines using Epstein-Barr virus
Method for generation of human B lymphoblastoid cell lines using Epstein-Barr virus
Lam, K.M.C.; Crawford, D.H.
Methods in Cell Science 17(2): 67-74
1995
ISSN/ISBN: 1381-5741
DOI: 10.1007/bf00986653
A detailed methodology is described for the successful immortalization of B lymphocytes to give lymphoblastoid cell lines using Epstein-Barr (EB) virus. A virus stock is prepared from B95-8 or Akata cell line, which spontaneously release high titers of virus, and the immortalizing titer of the preparation determined. B lymphocytes from fresh or cryopreserved samples are separated from the whole cell population, infected with EB virus and cultured for 4 weeks for the establishment of a lymphoblastoid cell line. The presence of EB virus encoded proteins in the immortalized cell line can be detected by indirect immunofluorescent staining.
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Accession: 009016518
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