Mitochondrial DNA mutations in Japanese detected by polymerase chain reaction--restriction fragment--single strand conformation polymorphism analysis

Tawata, M.; Ikegishi, Y.; Iwase, E.; Aida, K.; Onaya, T.

Genetic Analysis Biomolecular Engineering 14(1): 17-19

1997


PMID: 9158961
DOI: 10.1016/s1050-3862(97)01076-0
Accession: 009027823

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Abstract
PCR-restriction fragment-SSCP (PCR-RF-SSCP) analysis of mitochondrial DNA by HhaI/HincII in Japanese revealed 46 polymorphic patterns. The determinations of nucleotide sequence of these 46 patterns revealed 56 mutations compared with the Cambridge Sequence.