Modulation of nicotine on signal transduction of brain muscarinic acetylcholine receptors

Wang Hai, C.W.n Yu; Liu Chuan Gui

Zhongguo Yaolixue Yu Dulixue Zazhi 10(4): 241-246

1996


Accession: 009036901

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Abstract
The effects of nicotine on the signal transduction of rat brain muscarinic receptors were studied. Both the association and the dissociation kinetic processes of (3H)l-QNB binding to cerebral muscarinic receptors were slowed by preincubation with nicotine 1.0 mu-mol cntdot L-1. The processes of (35S)GTP-gamma-S binding with cerebral muscarinic receptors and G protein complexes were slowed by nicotine 1.0 mu-mol cntdot L-1, and the coupling relationship between the muscarinic receptors and G protein was potentiated. Nicotine 0.1-1000 mu-mol cntdot L-1 had no effects on the activity of adenylate cyclase in rat striatum. The levels of cytosolic calcium were increased by nicotine 0.1-30 mu-mol cntdot L-1 in a concentration-dependent manner. It is concluded that nicotine has multiple modulatory effects on the signal transduction of brain muscarinic receptors.