Modulatory role of platelet-derived growth factor on cytokine-induced nerve growth factor synthesis in rat glomerular mesangial cells
Plüss, K.M.; Pfeilschifter, J.; Mühl, H.; Huwiler, A.; Boeckh, C.; Otten, U.
Biochemical Journal 312: 707-711
ISSN/ISBN: 0264-6021 PMID: 8554509 DOI: 10.1042/bj3120707
Recent evidence indicates that cytokines are potent inducers of nerve growth factor (NGF) expression in peripheral tissues and in brain. Cultured rat glomerular mesangial cells respond to interleukin-1-beta (IL-1-beta) and turnout necrosis factor-alpha (TNF-alpha) by increased NGF synthesis. We found that co-stimulation of rat glomerular mesangial cells with platelet-derived growth factor (PGDF-BB) and IL-1-beta/TNF-alpha significantly augments the IL- 1-beta/TNF-alpha-induced NGF mRNA levels and NGF synthesis. In contrast, preincubation with PDGF-BB drastically reduces NGF gene expression and NGF protein synthesis in response to IL-1-beta/TNF-alpha stimulation. Thus our results indicate that PDGF-BB is a potent modulator of cytokine-induced NGF expression; its precise action is critically depending on the time at which the PDGF receptor is activated.