+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Molecular characterization of the transcription termination factor from human mitochondria



Molecular characterization of the transcription termination factor from human mitochondria



Journal of Biological Chemistry 268(11): 8123-8130



The transcription termination factor (mTERF), which plays a central role in the control of mitochondrial rRNA and mRNA synthesis in mammalian mitochondria, has been previously identified and purified by DNA affinity chromatography from a human mitochondrial lysate (Kruse, B., Narasimhan, N., and Attardi, G. (1989) Cell 58, 391-397). In the present work, this factor has been characterized as to its protein composition and the activities of the protein components. Three polypeptides, two of approximately 34-kDa molecular mass and one of approximately 31 kDa, were shown to be associated with the specific DNA binding and footprinting activity of the factor, with the 31-kDa component having a much lower affinity for the recognition sequence than the 34-kDa components. On the other hand, the transcription termination activity, as assayed in an in vitro system, was found to be associated exclusively with the two 34-kDa polypeptides. Mass spectroscopic analysis of tryptic peptides derived from highly purified polypeptides indicated that all three polypeptides share regions with common sequences. The evidence obtained suggests that differential phosphorylation is not responsible for the difference in electrophoretic mobility of the three polypeptides.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 009041440

Download citation: RISBibTeXText

PMID: 7681833


Related references

Termination of transcription in human mitochondria: identification and purification of a DNA binding protein factor that promotes termination. Cell 58(2): 391-397, 1989

DNA light-strand preferential recognition of human mitochondria transcription termination factor mTERF. Journal of Biochemistry and Molecular Biology 38(6): 690-694, 2005

Production, purification and characterization of mouse monoclonal antibodies against human mitochondrial transcription termination factor 2 (MTERF2). Protein Expression and Purification 82(1): 11-19, 2012

Mechanism of Transcription Anti-termination in Human Mitochondria. Cell 171(5): 1082-1093.E13, 2017

Rho dependent termination of transcription 1. identification and characterization of termination sites for transcription from the bacterio phage lambda p r promoter. Journal of Biological Chemistry 258(15): 9553-9564, 1983

Rho-dependent termination of transcription: identification and characterization of termination sites for transcription from the bacteriophage l PR promoter. The Journal of Biological Chemistry 258: 53-64, 1983

Phosphorylation of rat mitochondrial transcription termination factor (mTERF) is required for transcription termination but not for binding to DNA. Nucleic Acids Research 32(7): 2059-2068, 2004

Molecular characterization of the ets-related human transcription factor ER81. Oncogene 11(4): 771-779, 1995

Molecular cloning and characterization of a human DNA binding factor that represses transcription. Cell 59(5): 815-825, 1989

Purification and characterization of a transcription termination factor from vaccinia virions. Journal of Biological Chemistry 262(25): 12372-12380, 1987

Elongation factor NusG interacts with termination factor rho to regulate termination and antitermination of transcription. Genes and Development 7(1): 161-172, 1993

Thermodynamic and enzymological characterization of the interaction between transcription termination factor r and l cro mRNA. Biochemistry (American Chemical Society) 28: 10-17, 1989

Identification, molecular cloning, and characterization of the sixth subunit of human transcription factor TFIIIC. Journal of Biological Chemistry 282(23): 17179-17189, 2007

Thermodynamic and enzymological characterization of the interaction between transcription termination factor rho and lambda cro mRNA. Biochemistry 28(8): 3510-3517, 1989

In vitro characterization of transcription termination factor Rho from Escherichia coli rho(nusD) mutants. Journal of Molecular Biology 260(3): 332-346, 1996