+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Molecular cloning and nucleotide sequence of the gene encoding phosphate-inducible pectin lyase of Bacillus subtilis



Molecular cloning and nucleotide sequence of the gene encoding phosphate-inducible pectin lyase of Bacillus subtilis



Febs Letters. 398(2-3): 269-273



The gene encoding the pectin lyase (PNL; EC 4.2.2.10) of Bacillus subtilis has been cloned, sequenced, and characterized. A coding sequence for the PNL composed of 345 amino acids including a 24-amino-acid signal peptide was assigned. No sequence resembling a LexA binding site was found upstream of the structural gene. Furthermore, PNL activity of the gene product expressed in Escherichia coli DH5alpha was detected intracellularly, which might suggest that expression of the gene was not controlled by RecA. Regulation of the gene expression seemed to be quite different from that of other bacterial PNL genes previously reported.

(PDF emailed within 0-6 h: $19.90)

Accession: 009042483

Download citation: RISBibTeXText

PMID: 8977121

DOI: 10.1016/s0014-5793(96)01257-4


Related references

Molecular cloning, nucleotide sequence, and characterization of the Bacillus subtilis gene encoding the DNA-binding protein HBsu. Journal of Bacteriology 173(10): 3191-3198, 1991

Molecular cloning and nucleotide sequence of a pectin lyase gene from Pseudomonas marginalis N6301. Biochemical and biophysical research communications, 182(1): 14-19, 1992

Molecular cloning and analysis of nucleotide sequence of the bacillus subtilis lysa gene region using bacillus subtilis phage vectors and a multi copy plasmid pub110. Agricultural & Biological Chemistry 55(6): 1615-1626, 1991

Cloning and nucleotide sequence of the Bacillus subtilis trpB gene encoding tryptophan synthase b-subunit. Bioscience, Biotechnology, and Biochemistry 57: 06-9, 1993

Cloning and nucleotide sequence of the Bacillus subtilis K trpB gene encoding tryptophan synthase beta-subunit. Bioscience, Biotechnology, and Biochemistry 57(6): 1006-1009, 1993

Chromosomal location, cloning and nucleotide sequence of the Bacillus subtilis cdd gene encoding cytidine/deoxycytidine deaminase. Molecular & General Genetics 216(2-3): 462-468, 1989

Molecular cloning, DNA sequence, and expression of the gene encoding for thermostable pectate lyase of thermophilic Bacillus sp. TS 47. Bioscience, Biotechnology, and Biochemistry 65(2): 322-329, 2001

Molecular cloning and analysis of nucleotide sequence of the Bacillus subtilis lysA gene region using B. subtilis phage vectors and a multi-copy plasmid, pUB110. Agricultural and Biological Chemistry 55(6): 1615-1626, 1991

Nucleotide sequence and molecular characterization of pnlA, the structural gene for damage-inducible pectin lyase of Erwinia carotovora subsp. carotovora 71. Journal of Bacteriology 173(5): 1765-1769, 1991

Molecular cloning and nucleotide sequence of an endo-1,5-a- L-arabinase gene from Bacillus subtilis. European Journal of Biochemistry 245: 8-14, 1997

Molecular cloning and nucleotide sequence of the glycogen branching enzyme gene (glgB) from Bacillus stearothermophilus and expression in Escherichia coli and Bacillus subtilis. Molecular & General Genetics 230(1-2): 136-144, 1991

Molecular cloning and nucleotide sequence of an endo-1,5-alpha-L-arabinase gene from Bacillus subtilis. European Journal of Biochemistry 245(3): 708-714, 1997