Molecular cloning and sequencing of an alternatively spliced form of the human thyrotropin receptor transcript

Takeshita, A.; Nagayama, Y.; Fujiyama, K.; Yokoyama, N.; Namba, H.; Yamashita, S.; Izumi, M.; Nagataki, S.

Biochemical and Biophysical Research Communications 188(3): 1214-1219

1992


ISSN/ISBN: 0006-291X
PMID: 1445355
DOI: 10.1016/0006-291x(92)91360-3
Accession: 009042623

Download citation:  
Text
  |  
BibTeX
  |  
RIS

Article/Abstract emailed within 0-6 h
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Abstract
In the present study, we report the molecular cloning and sequence of an alternatively spliced form of the human thyrotropin receptor (hTSHR) mRNA transcript, which has previously been detected on Northern blot analysis of human thyroid cells. The smaller hTSHR cDNA, designated hTSHR cDNA-I, is approximately 1 kb in size and encodes a protein of 253 amino acids. Comparison of the nucleotide sequence of hTSHR cDNA-I with available hTSHR genomic sequence data reveals that the cDNA-I contains exons 1-98 and unidentified DNA tract, presumably an intron. Thus, the hTSHR cDNA-I encodes for the N-terminal half of the extracellular domain of the hTSHR (approximately 60%). The truncated TSHR-I may be secreted and function as a TSH binding protein.