EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Molecular genetic analysis of a prokaryotic transcriptional coactivator: functional domains of the bacteriophage T4 gene 33 protein



Molecular genetic analysis of a prokaryotic transcriptional coactivator: functional domains of the bacteriophage T4 gene 33 protein



Journal of Bacteriology 176(4): 1164-1171



The bacteriophage T4 gene 33 encodes a small, acidic RNA polymerase-binding protein that mediates enhancement of transcriptional initiation at T4 late promoters by the T4 DNA replication accessory proteins. A set of nested deletions in the gene 33 open reading frame was constructed by oligonucleotide site-directed mutagenesis. The resulting variant gene 33 proteins were radiolabeled during overexpression employing a T7 RNA polymerase-based system and substantially purified. Each variant was analyzed for three properties of gp33: RNA polymerase binding activity, ability to mediate enhancer-dependent transcriptional activation, and repression of unenhanced transcription. Two separate regions of gp33 were required to form stable complexes with RNA polymerase, whereas the extreme carboxyl terminus of gp33 was essential for mediating late gene activation. Variant gene 33 proteins lacking the carboxyl terminus nevertheless repressed nonenhanced transcription, demonstrating that the functional domains required for transcriptional activation and repression of unenhanced transcription are separable. The possible roles of gp33 in mediating late gene expression are discussed in the light of the identification of these functional domains.

(PDF emailed within 1 workday: $29.90)

Accession: 009045264

Download citation: RISBibTeXText

PMID: 8106327



Related references

Cloning and prokaryotic expression of transcriptional co-activator gene of Clonorchis sinensis and functional analysis of the expressed protein. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 23(1): 18-23, 2005

Identification, mutational analysis, and coactivator requirements of two distinct transcriptional activation domains of the Saccharomyces cerevisiae Hap4 protein. Eukaryotic Cell 3(2): 339-347, 2004

Cloning, prokaryotic expression and functional analysis of pep gene from Bacillus thuringiensis lysogenic bacteriophage. Wei Sheng Wu Xue Bao 48(4): 459-465, 2008

Molecular genetics of bacteriophage P22 scaffolding protein's functional domains. Journal of Molecular Biology 348(4): 831-844, 2005

Differential conservation of transcriptional domains of mammalian Prophet of Pit-1 proteins revealed by structural studies of the bovine gene and comparative functional analysis of the protein. Gene 291(1/2): 211-221, 2002

Molecular genetic analysis of bacteriophage P22 gene 3 product, a protein involved in the initiation of headful DNA packaging. Journal Of Molecular Biology. 227(4): 1086-1099, 1992

Molecular and biochemical studies on the DNA replication of bacteriophage T7: Functional analysis of amino-terminal region of gene 2.5 protein. Journal of Biochemistry and Molecular Biology 28(6): 484-489, 1995

Genetic and molecular analysis of an allelic series of cop1 mutants suggests functional roles for the multiple protein domains. Plant Cell 6(4): 487-500, 1994

Purification and properties of the bacteriophage P2 ogr gene product. A prokaryotic zinc-binding transcriptional activator. Journal of Biological Chemistry 265(13): 7472-7477, 1990

Major product pp43 of human cytomegalovirus U(L)112-113 gene is a transcriptional coactivator with two functionally distinct domains. Virology 260(1): 89-97, 1999

Genetic and molecular analysis of an allelic series of cop1 mutants suggests functional roles for the multiple protein domains: Plant Cell 6, 487500. Trends in Genetics 10(8): 263-0, 1994

Requirement for multiple domains of the protein arginine methyltransferase CARM1 in its transcriptional coactivator function. Journal of Biological Chemistry 277(48): 46066-46072, 2002

GRIP1, a novel mouse protein that serves as a transcriptional coactivator in yeast for the hormone binding domains of steroid receptors. Proceedings Of The National Academy Of Sciences Of The United States Of America. 93(10): 4948-4952, 1996

Molecular genetic evidence for functional domains within the vpi gene product. Plant Physiology (Rockville) 89(4 SUPPL): 139, 1989

The Transcriptional Coactivator CREB-binding Protein Cooperates with STAT1 and NF-kB for Synergistic Transcriptional Activation of the CXC Ligand 9/Monokine Induced by Interferon-c Gene. The Journal of Biological Chemistry 278(1): 1-60, 2002