EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

NSF is required for transport from early to late endosomes


NSF is required for transport from early to late endosomes



Journal of Cell Science 110: 2079-2087



ISSN/ISBN: 0021-9533

PMID: 9378758

Protein transport between early and late endosomes is a major membrane trafficking pathway in the cell followed by many proteins, including all down-regulated receptors. Yet, little is known at the molecular level about the mechanisms regulating membrane interactions in the endocytic pathway beyond early endosomes. In this study, we have used an in vitro transport assay to study the biochemical properties of endosome docking/fusion events. Our data demonstrate that N-ethylmaleimide (NEM) sensitive factor (NSF) and its soluble associated proteins (SNAPs) are required for transport from early to late endosomes, as well as at all other steps of endosomal membrane transport. We also find that these proteins are enriched on endosomal membranes. In addition, our studies suggest that besides NSF/SNAPs, another NEM-sensitive component may also be involved in docking/fusion at this late stage of the pathway. Finally, we find that, in contrast to Golgi membranes, NSF association to both early and late endosomal membranes occurs via an ATP-independent mechanism, indicating that the binding properties of endosomal and biosynthetic NSF are different. Our data thus show that NSF/SNAPs, perhaps together with another NEM-sensitive factor, are part of the basic molecular machinery which controls docking/fusion events during transport from early to late endosomes, along the lysosomal degradation pathway.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 009075198

Download citation: RISBibTeXText

Related references

Rab11 regulates the compartmentalization of early endosomes required for efficient transport from early endosomes to the trans-golgi network. Journal of Cell Biology 151(6): 1207-1220, 2000

End13p/Vps4p is required for efficient transport from early to late endosomes in Saccharomyces cerevisiae. Journal of Cell Science 114(Pt 10): 1935-1947, 2001

Rab7 associates with early endosomes to mediate sorting and transport of Semliki forest virus to late endosomes. Plos Biology 3(7): e233, 2005

Transport to late endosomes is required for efficient reovirus infection. Journal of Virology 86(16): 8346-8358, 2012

Membrane transport from early to late endosomes. Cold Spring Harbor Symposia on Quantitative Biology 60: 205-209, 1995

CLIP-170 is essential for transport between early and late endosomes. European Journal of Cell Biology 69(Suppl. 42): 80, 1996

ESCRT-I function is required for Tyrp1 transport from early endosomes to the melanosome limiting membrane. Traffic 10(9): 1318-1336, 2009

Early Endosomes Associated with Dynamic F-actin Structures Are Required for Late Trafficking of H. pylori VacA Toxin. The Journal of Cell Biology 177(2): 343-354, 2007

Early endosomes associated with dynamic F-actin structures are required for late trafficking of H. pylori VacA toxin. Journal of Cell Biology 177(2): 343-354, 2007

Infectious adenovirus type 2 transport through early but not late endosomes. Traffic 9(12): 2265-2278, 2008

Early endosomes are required for major histocompatiblity complex class II transport to peptide-loading compartments. Molecular Biology of the Cell 10(9): 2891-2904, 1999

Efficient transport of avidin fusion proteins into late and early endosomes. Molecular Biology of the Cell 7(Suppl. ): 452A, 1996

Cytoplasmic dynein-dependent vesicular transport from early to late endosomes. Journal of Cell Biology 123(6 Pt 1): 1373-1387, 1993

Invariant chain induces a delayed transport from early to late endosomes. Journal of Biological Chemistry 270(6): 2741-2746, 1995

The golgin GCC88 is required for efficient retrograde transport of cargo from the early endosomes to the trans-Golgi network. Molecular Biology of the Cell 18(12): 4979-4991, 2007