+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Neurite extension after damage by laser beam irradiation in cultured chick sensory neurons



Neurite extension after damage by laser beam irradiation in cultured chick sensory neurons



Biological and Pharmaceutical Bulletin 17(11): 1477-1480



We studied the effects of neurotrophic factors on neurite extension and branching of embryonic chick sensory neurons in culture after damage to their filopodia by an irradiating laser beam. The factors employed were nerve growth factor (NGF), basic fibroblast growth factor (bFGF), ganglioside GM1 (GM1), laminin (LAM), and combinations of bFGF plus NGF, and LAM plus NGF. Neither NGF, bFGF, nor NGF plus bFGF affected either the neurite extension or the amount of branching of damaged and intact neurites. Ten micrograms/ml of GM1 tended to enhance the extension of damaged neurites. One hundred micrograms/ml of LAM significantly enhanced the elongation of damaged neurites but not intact ones. One hundred micrograms/ml of LAM with 10 ng/ml of NGF significantly elongated the neurite lengths of both damaged and intact neurites. These results suggest that LAM plays an important role in neurite extension after damage to peripheral sensory neurons and that NGF modulates the effect of LAM.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009083749

Download citation: RISBibTeXText

PMID: 7703967

DOI: 10.1248/bpb.17.1477


Related references

Developmental decrease in neurite extension in cultured chick embryo retina and spinal cord neurons. Developmental Biology 84(1): 244-246, 1981

Neuro-p24 plays an essential role in neurite extension: antisense oligonucleotide inhibition of neurite extension in cultured DRG neurons and neuroblastoma cells. Neuroscience Research 50(2): 199-208, 2004

Radicicol potentiates neurotrophin-mediated neurite outgrowth and survival of cultured sensory neurons from chick embryo. Journal of Neurochemistry 72(6): 2256-2263, 1999

Laser inactivation of pp60-c-src increases the rate of neurite extension in chick dorsal root ganglia neurons. Society for Neuroscience Abstracts 23(1-2): 1414, 1997

Cholinesterase inhibitors induced growth cone collapse and inhibit neurite extension in primary cultured chick neurons. Neurotoxicology & Teratology 20(4): 411-419, 1998

Insulin and somatemedin MSA promote nerve growth factor-independent neurite formation by cultured chick dorsal root ganglionic sensory neurons. Journal of Neuroscience Research 8(2-3): 225-231, 1982

Insulin and somatomedin multiplication stimulating activity promote nerve growth factor independent neurite formation by cultured chick dorsal root ganglionic sensory neurons. Journal of Neuroscience Research 8(2-3): 225-232, 1982

Purified chick DM-GRASP supports neurite extension from A subpopulation of neurons in vitro. Society for Neuroscience Abstracts 19(1-3): 1091, 1993

Tumor necrosis factor-α elevates neurite outgrowth through an NF-κB-dependent pathway in cultured adult sensory neurons: Diminished expression in diabetes may contribute to sensory neuropathy. Brain Research 1423: 87-95, 2011

Monoclonal M6 antibody interferes with neurite extension of cultured neurons. Journal of Neurobiology 23(1): 71-88, 1992

Segmental independence and age dependence of neurite outgrowth from embryonic chick sensory neurons. Journal of Neurobiology 26(1): 1-16, 1995

Tau is required for neurite outgrowth and growth cone motility of chick sensory neurons. Cell Motility and the Cytoskeleton 43(3): 232-242, 1999

Cholinergic modulation of neurofilament expression and neurite outgrowth in chick sensory neurons. Journal of Neuroscience Research 73(2): 227-234, 2003

Movement of embryonic chick sympathetic neurons on laminin in vitro is preceded by neurite extension. Journal of Neuroscience Research 36(6): 607-620, 1993

Attachment, survival and neurite extension of chick embryo retinal neurons on various culture substrates. Developmental Neuroscience 5(5-6): 447-457, 1982