Novel 7-hydroxycoumarin based fluorescent labels
Adamczyk, M.; Cornwell, M.; Huss, J.; Rege, S.; Rao, T.V.nkata S.
Bioorganic and Medicinal Chemistry Letters 7(15): -1988
Five new 7-hydroxy-8-(N-alkylaminomethyl)coumarin labels (6a-e) were prepared from 4-(4-carboxybutyl)-7-hydroxycoumarin (4) in 28-48% yield. Compared to 4-methylumbelliferone (4-MU, 1), the new fluorescent labels exhibited higher emission intensity over a broader pH range. Extinction coefficients, quantum yields and pKa values are also reported for these labels.