+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Optimization of hypothermosol for the hypothermic storage of cardiac cells - addition of EDTA

Optimization of hypothermosol for the hypothermic storage of cardiac cells - addition of EDTA

In Vitro Toxicology 10(4): 407-415, Winter

Hypothermic solutions have been developed for shipping and storing organs, tissues, and cells. Cells are immersed in these solutions and kept at 4degreeC where they remain in a state of near suspended animation for long periods of time. A number of modifications to current cold-storage solutions have been suggested that improve the ability of these media to protect cells during hypothermic storage. We show that the Ca2+/Mg2+ chelator, ethylenediaminetetraacetic acid (EDTA), can confer added protection to a mixed chick cardiac cell population stored in the cold-storage solution, HypoThermosol. Both the base HypoThermosol and the EDTA-supplemented HypoThermosol are better at protecting the cardiac cells from cold-induced damage than is ViaSpan (University of Wisconsin (UW) solution). To further understand why EDTA confers a protective effect, cardiac cells were cold-stored in HypoThermosol containing 5 mM Mg2+ and 0 mM Ca2+ in the presence or absence of EDTA. HypoThermosol containing 0 mM Ca2+, 5 mM Mg2+, and no EDTA was less effective as a cold-storage solution than the base HypoThermosol solution that contained 0.05 mM Ca2+ and 5 mM Mg2+; whereas in HypoThermosol containing 0 mM Ca2+, 5 mM Mg2+, and EDTA was the best at preserving the cardiac cells. When cardiac cells were stored in the base HypoThermosol solution containing 0.05 mM Ca2+ and 5 mM Mg2+, there was a time dependent leakage of Ca2+ from the cells that peaked at 7 days of cold storage. Therefore, we conclude that the positive effect conferred by EDTA on the ability of HypoThermosol to act as a cold-storage solution might be due to its ability to sequester intracellular calcium (Cai2+) that leaks from the cells and accumulates as Ca2+ during cold storage. The fact that a similar EDTA enhancing effect is seen in the cold storage of other cells suggests that the leakage of Cai2+ should be seriously considered in the design of future cold-storage solutions.

(PDF emailed within 1 workday: $29.90)

Accession: 009135067

Download citation: RISBibTeXText

Related references

Hypothermic storage of isolated human hepatocytes: a comparison between University of Wisconsin solution and a hypothermosol platform. Archives of Toxicology 83(5): 493-502, 2009

Hypothermic preservation of immature spinal cord using hypothermosol for transplantation in the rat. Cryobiology 33(6): 675, 1996

Vitamin E and EDTA Improve the Efficacy of Hypothermosol-Implication of Apoptosis. In Vitro & Molecular Toxicology 12(3): 163-172, 2000

Static cold storage of isolated rabbit hearts in hypothermosol Influence of butanedione monoxime during short-term storage. Cryobiology 32(6): 538-539, 1995

Optimization of hypothermosol using cultured cardiomyocytes and the fluorescent multiple endpoint assay. Cryobiology 32(6): 590-591, 1995

Cold-Storage of Synthetic Human Epidermis in HypoThermosol. Tissue Engineering 1(4): 361-377, 1995

Preservation of rat livers by cold storage: a comparison between the University of Wisconsin solution and Hypothermosol. Annals of Transplantation 9(2): 35-37, 2004

The effect of buffer and ph on hypothermic storage of the cardiac explant. Journal of Molecular & Cellular Cardiology 21(SUPPL 2): S158, 1989

The calcium requirement for hypothermic storage of the cardiac explant. Current Surgery 46(4): 313-316, 1989

Continuous cardiac perfusion preservation with PEG-HB improves cardiac recovery from ischemic insult over standard ischemic hypothermic storage. Journal of Investigative Medicine 48(1): 32A, January, 2000

Cardiac function and myocardial energy level after prolonged hypothermic storage. Transplantation Proceedings 30(7): 3331-3333, 1998

Long-term hypothermic storage of the cardiac explant. Comparison of four solutions. Journal of Cardiovascular Surgery 32(1): 21-25, 1991

Improved cardiac functional recovery with Na+H+ exchange inhibitor Hoe 694 following hypothermic storage. Cryobiology 35(4): 345-346, 1997

Improved recovery of cardiac function after hypothermic ischemic storage with ouabain. Journal of Thoracic and Cardiovascular Surgery 96(5): 782-788, 1988

The individual and combined protective effects of aspartate and glutamate during hypothermic cardiac storage. Journal of Molecular & Cellular Cardiology 24(11): XV, 1992