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Optimization of the determination of inorganic and organic selenium species using high-performance liquid chromatography-electrothermal atomic absorption spectrometry



Optimization of the determination of inorganic and organic selenium species using high-performance liquid chromatography-electrothermal atomic absorption spectrometry



Journal Of Chromatography A. 750(1-2): 327-334



The liquid chromatographic simultaneous separation of selenite, selenate, selenocystine and selenomethionine was studied with a selenium-specific detector; electrothermal atomic absorption spectrometry. Three chromatographic modes were compared; two ion-pairing ones using either a cationic or an anionic reagent and ion-exchange. Separation parameters such as composition, pH and concentration of the mobile phase were investigated. The influence of chromatographic conditions on the sensitivity of the detector is studied. The ion-exchange method was determined to give the best results, with detection limits ranging from 8 to 17 mu-g l-1 within a 30 min separation time. An application of this method to the analysis of seleno compounds present in a selenium-rich yeast after enzymic hydrolysis extraction is presented.

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Accession: 009135360

Download citation: RISBibTeXText

DOI: 10.1016/0021-9673(96)00484-0


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