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Permeability of the Escherichia coli outer membrane to ethidium ions and periplasmic alkaline phosphatase during enhanced synthesis of the alkaline phosphatase

Permeability of the Escherichia coli outer membrane to ethidium ions and periplasmic alkaline phosphatase during enhanced synthesis of the alkaline phosphatase

Biochemistry (Moscow) 60(7): 881-887

The outer membrane of various Escherichia coli strains overproducing periplasmic alkaline phosphatase was found to be permeable not only to the enzyme, but also to ethidium ions, which normally do not penetrate into gram-negative bacteria. In the absence of the outer membrane lipoprotein, the permeability of the membrane to these compounds and its sensitivity to membranotropic agents increase, indicating a role of the lipoprotein in the barrier properties of the outer membrane. A negative correlation was revealed between the contents of lipoprotein and lipopolysaccharide in the outer membrane and between their content and alkaline phosphatase secretion into the medium. It is suggested that the increased permeability of the Escherichia coli outer membrane during overproduction of the secreted protein is due to impaired biogenesis of membrane components.

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