EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Permeability of the Escherichia coli outer membrane to ethidium ions and periplasmic alkaline phosphatase during enhanced synthesis of the alkaline phosphatase


Permeability of the Escherichia coli outer membrane to ethidium ions and periplasmic alkaline phosphatase during enhanced synthesis of the alkaline phosphatase



Biochemistry (Moscow) 60(7): 881-887



The outer membrane of various Escherichia coli strains overproducing periplasmic alkaline phosphatase was found to be permeable not only to the enzyme, but also to ethidium ions, which normally do not penetrate into gram-negative bacteria. In the absence of the outer membrane lipoprotein, the permeability of the membrane to these compounds and its sensitivity to membranotropic agents increase, indicating a role of the lipoprotein in the barrier properties of the outer membrane. A negative correlation was revealed between the contents of lipoprotein and lipopolysaccharide in the outer membrane and between their content and alkaline phosphatase secretion into the medium. It is suggested that the increased permeability of the Escherichia coli outer membrane during overproduction of the secreted protein is due to impaired biogenesis of membrane components.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 009174854

Download citation: RISBibTeXText

Related references

Permeability of the Escherichia coli outer membrane for ethidium ions and periplasmic alkaline phosphatase during increased synthesis of it. Biokhimiia 60(7): 1161-1170, 1995

Importance of membrane fluidity in the induction of alkaline phosphatase, a periplasmic enzyme, in Escherichia coli. Biochemical and Biophysical Research Communications 70(3): 900-906, 1976

Comparative study of properties of periplasmic and membrane bound alkaline phosphatase of escherichia coli. Biokhimiya 46(7): 1249-1257, 1981

Membrane bound precursor of escherichia coli periplasmic alkaline phosphatase isolation and some properties. Molekulyarnaya Biologiya 15(1): 104-114, 1981

Synthesis of alkaline phosphatase by membrane fractions of escherichia coli the effect of rna polymerase and of dna isolated from phosphatase positive strain. Annales de l'Institut Pasteur (Paris) 118(5): 597-610, 1970

Comparative study of the properties of the periplasmic and membrane bound alkaline phosphatase ec 3.1.3.1 of escherichia coli. Biochemistry 46(7 Part 2): 1006-1014, 1981

Membrane phospholipid composition of Escherichia coli affects secretion of periplasmic alkaline phosphatase into the medium. Biochemistry. Biokhimiia 68(7): 752-759, 2003

Synthesis of alkaline phosphatase by membrane fractions of Escherichia coli. Effect of addition of RNA-polymerase and DNA arising from "phosphatase-positive" strains. Annales de l'Institut Pasteur 118(5): 597-610, 1970

Outer membrane protein e of Escherichia coli K-12 is co-regulated with alkaline phosphatase. Journal of Bacteriology 143(1): 151-157, 1980

Escherichia coli signal peptides direct inefficient secretion of an outer membrane protein (OmpA) and periplasmic proteins (maltose-binding protein, ribose-binding protein, and alkaline phosphatase) in Bacillus subtilis. Journal of Bacteriology 176(10): 3013-3020, 1994

Excretion of alkaline phosphatase by Escherichia coli K-12 pho constitutive mutants transformed with plasmids carrying the alkaline phosphatase structural gene. Journal of Bacteriology 164(3): 1376-1380, 1985

Conversion of escherichia coli alkaline phosphatase into lipoprotein by the introduction of leu ala gly cys into the signal sequence of alkaline phosphatase precursor. Abstracts of the Annual Meeting of the American Society for Microbiology 88: 220, 1988

Study of the biogenesis and secretion of alkaline phosphatase and its mutant forms in Escherichia coli. I. Introduction of directed mutations into the alkaline phosphatase gene. Molekuliarnaia Biologiia 28(1): 150-157, 1994

Functional interrelationships in the alkaline phosphatase superfamily: phosphodiesterase activity of Escherichia coli alkaline phosphatase. Biochemistry 40(19): 5691-5699, 2001

Bacillus subtilis alkaline phosphatases III and IV. Cloning, sequencing, and comparisons of deduced amino acid sequence with Escherichia coli alkaline phosphatase three-dimensional structure. Journal of Biological Chemistry 266(2): 1077-1084, 1991