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Plant regeneration from suspension cultures of Allium fistulosum and an A. fistulosum X A. cepa interspecific hybrid



Plant regeneration from suspension cultures of Allium fistulosum and an A. fistulosum X A. cepa interspecific hybrid



Plant Science 98(1): 63-68



Suspension cultures were initiated from friable nodule-forming calluses of five onion genotypes, two of Allium fistulosum L. and three of A. cepa L., and an interspecific A. fistulosum times A. cepa F-1 diploid hybrid. Eight liquid BDS (basal Dunstan and Short (2)) media supplemented with factorial combinations of plant growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D), abscisic acid (ABA), or 6-benzylaminopurine (6-BAP) were used for initiating suspension cultures. More suspension cultures were initiated from A. fistulosum cultivars (30.0%) and the interspecific A. fistulosum times A. cepa diploid F-1 hybrid (25.0%) than from A. cepa cultivars (21.6%). Cell clumps cultured in liquid BDS medium with 0.5 mg/l ABA and 1.0 or 2.0 mg/l 2,4-D without 6-BAP were more finely dispersed than clumps cultured with 6-BAP in all genotypes. It was difficult to recover shoots from suspension cultures of A. cepa, but clumps from suspension cultures of the hybrid and A. fistulosum regenerated shoots which subsequently developed into plants.

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Related references

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