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Plantlets from encapsulated micropropagated buds of M.26 apple rootstock



Plantlets from encapsulated micropropagated buds of M.26 apple rootstock



Plant Cell Tissue and Organ Culture 47(3): 255-260



In order to be considered usable as synthetic seeds, encapsulated explants sown under in vitro or ex vitro conditions must be able to produce whole plantlets. Ninety percent of non-encapsulated M.26 apple rootstock single nodes produced a plantlet (i.e., a well-formed shoot with a root system) after 30 days of culture in vitro if the explants were previously given a 24-hour treatment with 24.6 micromolar IBA and 15 g l -1 sucrose in darkness. In contrast, when the single nodes were encapsulated in a calcium-sodium alginate bead immediately after the same treatment only 10% of the encapsulated explants formed a plantlet. Addition of growth regulators to the artificial endosperm and culture of the single nodes for root primordia initiation for 3, 6 or 9 days in darkness before encapsulation allowed production of 58%, 60% and 66% of plantlets, respectively.

Accession: 009202076

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DOI: 10.1007/bf02318980

Download PDF Full Text: Plantlets from encapsulated micropropagated buds of M.26 apple rootstock



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