+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Poly(ethylene glycol) limits of detection using internal matrix-assisted laser desorption/ionization Fourier transform mass spectrometry



Poly(ethylene glycol) limits of detection using internal matrix-assisted laser desorption/ionization Fourier transform mass spectrometry



Journal of Mass Spectrometry 33(5): 473-479



Detection limits of poly(ethylene glycol) were examined in the mass range 2000-6000 Da. Using an aerospray sample deposition technique, highly uniform sample surfaces were produced. This method allows signal averaging of spectra from up to 400 shots on the same sample spot. It is found that, as the material available for desorption is decreased, the overall average sample consumption per shot is decreased. Experimentally determined detection limits of 40 and 280 fmol (based on the average molecular masses of 2000 and 6000) were found for PEG 2000 and PEG 6000, respectively. The sample spectra show oligomer distributions in agreement with their higher concentration counterparts. However, at the lowest signal-to-noise levels, oligomers at the extremes of the distribution are no longer detected, making the polymer distribution appear to be narrower in mass range.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009209392

Download citation: RISBibTeXText

PMID: 9615419

DOI: 10.1002/(sici)1096-9888(199805)33:5<473::aid-jms659>3.0.co;2-o


Related references

The characterization of dendronized poly(ethylene glycol)s and poly(ethylene glycol) multi-arm stars using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Analytica Chimica Acta 808: 175-189, 2014

Importance of matrix:analyte ratio for buffer tolerance using 2,5-dihydroxybenzoic acid as a matrix in matrix-assisted laser desorption/ionization-Fourier transform mass spectrometry and matrix-assisted laser desorption/ionization-time of flight. Journal of the American Society for Mass Spectrometry 9(8): 805-813, 1998

Evaluation of combined matrix-assisted laser desorption/ionization time-of-flight and matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry experiments for peptide mass fingerprinting analysis. Rapid Communications in Mass Spectrometry 25(13): 1881-1892, 2011

Endgroup analysis of polyethylene glycol polymers by matrix-assisted laser desorption/ionization Fourier-transform ion cyclotron resonance mass spectrometry. Rapid Communications in Mass Spectrometry 9(10): 957-962, 1995

How Suitable is Matrix-Assisted Laser Desorption/Ionization-Time-of-Flight for Metabolite Imaging from Clinical Formalin-Fixed and Paraffin-Embedded Tissue Samples in Comparison to Matrix-Assisted Laser Desorption/Ionization-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry?. Analytical Chemistry 88(10): 5281-5289, 2016

A Novel Derivatization Reagent in the Determination of the Number of OH End Groups in Poly(ethylene glycol) by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. Mass Spectrometry 2(1): A0022-A0022, 2013

Flexible open-cell design for internal-source matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry. Rapid Communications in Mass Spectrometry 15(12): 979-985, 2001

Absolute internal mass calibration with carbon soot ions using high-resolution matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry. Rapid Communications in Mass Spectrometry 28(19): 2147-2150, 2014

Effects of different alkali metal ions on the cationization of poly(ethylene glycol)s in matrix-assisted laser desorption/ionization mass spectrometry: a new selectivity parameter. Journal of Mass Spectrometry 37(10): 1074-1080, 2002

Comparison of laser desorption and matrix-assisted laser desorption/ionization for ruthenium and osmium trisbipyridine complexes using Fourier transform mass spectrometry. Journal of the American Society for Mass Spectrometry 14(4): 393-400, 2003

Matrix-assisted laser desorption ionization Fourier-transform mass spectrometry. Rapid Communications in Mass Spectrometry 8(6): 498-500, 1994

Assisted inhibition effect of acetylcholinesterase with n-octylphosphonic acid and application in high sensitive detection of organophosphorous pesticides by matrix-assisted laser desorption/ionization Fourier transform mass spectrometry. Analytica Chimica Acta 706(2): 291-296, 2012

Evaluation of the quantitativeness of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using an equimolar mixture of uniform poly(ethylene glycol) oligomers. Journal of Mass Spectrometry 38(9): 948-954, 2003

Matrix-assisted laser desorption/ionization Fourier-transform mass spectrometry of oligodeoxyribonucleotides. Rapid Communications in Mass Spectrometry 7(9): 828-836, 1993

Limits for the detection of (poly-)phosphoinositides by matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI-TOF MS). Chemistry and Physics of Lipids 110(2): 151-164, 2001