+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Potentiation by higenamine of the aconitine-induced positive chronotropic effect in isolated right atria of mice: the effects of cholera toxin, forskolin and pertussis toxin



Potentiation by higenamine of the aconitine-induced positive chronotropic effect in isolated right atria of mice: the effects of cholera toxin, forskolin and pertussis toxin



Biological and Pharmaceutical Bulletin 19(8): 1032-1037



Aconitine and higenamine are the major cardioactive compounds obtained from processed aconite. The chronotropic interaction between these two compounds was investigated in isolated right atria of mice. Both aconitine and higenamine potentiated the action of the other. Practolol (1 nM), a selective beta 1-adrenergic antagonist, but not butoxamine (1 microM), a beta 2-adrenergic antagonist, blocked the potentiation by higenamine (5 nM) of the aconitine-induced positive chronotropic effect and, at high concentrations (30 and 300 nM) also shifted the aconitine concentration-response curves to the right. The potentiating interaction between aconitine and higenamine was reversed by pretreating with cholera toxin (CTX) and forskolin. In CTX (100 nM, 1 h)- and forskolin (30 and 100 nM)-treated atria, higenamine significantly depressed the aconitine-induced response, which was abolished by pertussis toxin (PTX, 150 micrograms/kg, i.p., 3 d). Neither CTX (50 and 100 nM) nor forskolin (15-100 nM) significantly affected the aconitine-induced positive chronotropic effect, while PTX (150 micrograms/kg) depressed it. These results suggest that the potentiating interaction between aconitine and higenamine involves "cross-talk" between the beta 1-adrenergic signalling pathway and Gi-protein.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009223590

Download citation: RISBibTeXText

PMID: 8874810

DOI: 10.1248/bpb.19.1032


Related references

Cholera toxin accentuates the antagonism by acetylcholine of higenamine-induced positive chronotropy in isolated right atria of mice. Biological & Pharmaceutical Bulletin 18(11): 1509-1512, 1995

Positive chronotropic and inotropic effects of higenamine and its enhancing action on the aconitine-induced tachyarrhythmia in isolated murine atria. Japanese Journal of Pharmacology 66(1): 75-80, 1994

Pertussis toxin actions on the pituitary-derived 235-1 clone: effects of PGE1, cholera toxin, and forskolin on cyclic AMP metabolism and prolactin release. Journal of Cyclic Nucleotide and Protein Phosphorylation Research 9(3): 245-258, 1983

Effects of follicle stimulating hormone cholera toxin pertussis toxin and forskolin on 3' 5' cyclic amp output by granulosa cells from booroola ewes with or without the f gene. Journal of Reproduction and Fertility 89(2): 553-564, 1990

Effects of follicle stimulating hormone, cholera toxin, pertussis toxin and forskolin on adenosine cyclic 3',5'-monophosphate output by granulosa cells from Booroola ewes with or without the F gene. Journal of Reproduction and Fertility 89(2): 553-563, 1990

Autoregulation of acute progesterone and adenosine 3',5'-monophosphate responses to follicle-stimulating hormone (FSH) in porcine granulosa cells: effects of FSH, cholera toxin, forskolin, and pertussis toxin. Endocrinology 123(5): 2367-2373, 1988

Luteinizing Hormone Secretion Is Enhanced by Pertussis Toxin, Cholera Toxin, and Forskolin. Neuroendocrinology 37(2): 161-163, 1983

Human pancreatic tumor growth hormone (GH) - releasing factor and cyclic adenosine 3',5'- monophosphate evoke GH release from anterior pituitary cells: the effects of pertussis toxin, cholera toxin, forskolin, and cycloheximide. Endocrinology 114(3): 904-913, 1984

Regulation of bradykinin receptor level by cholera toxin pertussis toxin and forskolin in cultured human fibroblasts. Journal of Cell Biology 109(4 PART 2): 150A, 1989

Regulation of bradykinin receptor level by cholera toxin, pertussis toxin and forskolin in cultured human fibroblasts. British Journal of Pharmacology 103(2): 1347-1350, 1991

Luteinizing hormone secretion is enhanced by pertussis toxin, cholera toxin, and forskolin. Evidence for the involvement of the cyclic AMP-generating system. Neuroendocrinology 37(2): 161-163, 1983

Identification of gtp binding proteins by adp ribosylation in the presence of cholera toxin pertussis toxin and botulinum toxin d in plasma membrane isolated from eggs and embryos of sea urchin. Development Growth & Differentiation 34(2): 211-222, 1992

Differential modulatory roles of cholera toxin and pertussis toxin in the regulation of pain responses induced by excitatory amino acids administered intrathecally in mice. Brain Research 867(1-2): 246-249, 9 June, 2000

Effects of pertussis toxin and cholera toxin on the platelet activating factor paf induced prostaglandin synthesis in cultured rat kupffer cells. FASEB Journal 3(4): 1224, 1989

The effects of pertussis toxin and cholera toxin on mitogen-induced interleukin-2 production: evidence for G protein involvement in signal transduction. Cellular Immunology 113(2): 235-250, 1988