+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Preimplantation development of mouse embryos in KSOM: augmentation by amino acids and analysis of gene expression

Preimplantation development of mouse embryos in KSOM: augmentation by amino acids and analysis of gene expression

Molecular Reproduction and Development 41(2): 232-238

ISSN/ISBN: 1040-452X

PMID: 7654376

DOI: 10.1002/mrd.1080410214

Simplex optimization has generated several media that have improved the development of mouse preimplantation embryos in vitro. One objective of this study was to compare the development of preimplantation mouse embryos in one of these computer-optimized media, KSOM, with embryos that developed in vivo, in terms of the relative abundances of specific mRNAs involved in metabolism, transcription, and cell proliferation. First, however, since studies have indicated an improvement of other simple embryo culture media by addition of amino acids, the effects of the addition of amino acids to KSOM (KSOM/AA) on preimplantation development were assessed. We find that addition of both essential and nonessential amino acids to KSOM augments development in vitro, as compared to development supported by KSOM without amino acids. This augmentation is observed starting at the blastocyst stage, and is associated with increased rate of development to the blastocyst stage, increased frequency of hatching, and increased number of cells in the blastocysts. Reverse-transcription PCR was then used to assess the relative abundance of mRNAs for actin, glyceraldehyde-3-phosphate dehydrogenase, Na+, K(+)-ATPase, Sp1, TATA box-binding protein TBP, IGF-I, IGF-II, IGF-I receptor, and IGF-II receptor in embryos that developed in vivo and in vitro using KSOM/AA. Eight out of 9 of these mRNAs were present in the 8-cell embryos and blastocysts raised in KSOM/AA in amounts that were indistinguishable from those in embryos that developed in vivo. It is concluded that KSOM/AA provides an environment in which preimplantation mouse embryos can undergo development that is quantitatively similar to that occurring in vivo.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 009231284

Download citation: RISBibTeXText

Related references

Analysis of Qa-2 antigen expression by preimplantation mouse embryos: possible relationship to the preimplantation-embryo-development (Ped) gene product. Biology of Reproduction 36(3): 611-616, 1987

Comparative analysis of development-related gene expression in mouse preimplantation embryos with different developmental potential. Molecular Reproduction and Development 72(2): 152-160, 2005

Differential expression of G6PDH mRNA transcripts during Kunming mouse preimplantation embryo development in CZB and KSOM. Chinese Journal of Veterinary Science 22(4): 359-362, 2002

Effect of amino acids and alpha-amanitin on the development of rabbit embryos in modified protein-free KSOM with HEPES. Molecular Reproduction and Development 45(2): 157-162, 1996

Effect of 5-azacytidine on gene expression and development of preimplantation mouse embryos. Biology of Reproduction 56(Suppl. 1): 188, 1997

Effect of ICSI on gene expression and development of mouse preimplantation embryos. Human Reproduction 25(12): 3012-3024, 2010

Effect of ICSI on gene expression and development of mouse preimplantation embryos. Human Reproduction 28(1): 285-285, 2013

Comparison of gene expression during preimplantation development between diploid and haploid mouse embryos. Biology of Reproduction 67(2): 386-392, 2002

Development and imprinted gene expression in uniparental preimplantation mouse embryos in vitro. Molecular Biology Reports 42(2): 345-353, 2015

Effect of in vitro fertilization on gene expression and development of mouse preimplantation embryos. Reproduction 134(1): 63-72, 2007

Effect of RNA Interference Hsp72 Gene Expression on Development of Mouse Preimplantation Embryos. Agricultural Sciences in China 8(2): 223-230, 2009

Analysis of thymidine kinase gene expression in preimplantation mouse embryos. Molecular Reproduction and Development 39(3): 259-267, 1994

Mouse embryonic development from one-cell stage to blastocyst, utilizing KSOM supplemented with amino acids in a microchannel device. Reproduction Fertility and Development 16(1-2): 205, 2004

Effects of glucose and cysteine added to a serum-free medium supplemented with a nitric oxide scavenger and amino acids on preimplantation development of ICR mouse 1-cell embryos. Theriogenology 53(1): 298, 2000

Optimization of real time RT-PCR methods for the analysis of gene expression in mouse eggs and preimplantation embryos. Molecular Reproduction and Development 71(3): 284-289, 2005