+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Prevalence of precore-defective mutant of hepatitis B virus in HBV carriers



Prevalence of precore-defective mutant of hepatitis B virus in HBV carriers



Journal of Medical Virology 46(4): 397-402



Two hundred and seventy-three serum specimens from hepatitis B virus (HBV) carriers were examined for the presence of a characteristic one point mutation at nucleotide (nt) 1896 from the EcoRI site of the HBV genome in the precore region (the preC mutant) using restriction fragment length polymorphism (RFLP) analysis. This assay approach could detect preC mutants or wild-type sequences when either form constituted more than 10% of the total sample. Overall, 65.5% (76/116) of HBeAg-positive carriers had only the preC wild-type. All HBeAg-positive asymptomatic carriers (n = 14) had only the preC wild-type. In patients with chronic hepatitis B and in anti-HBe-positive asymptomatic carriers, increased prevalence of the preC mutant was associated with the development of anti-HBe antibodies and normalization of the serum alanine aminotransferase concentration. Furthermore, 27 (29.0%) of 93 HBeAg-negative carriers had unexpectedly preC wild-type sequences only. Direct sequencing of the HBV precore region of HBV specimens from 24 patients revealed no mutation at nt 1896, supporting the specificity of the RFLP analysis. These results suggest that RFLP analysis was accurate for the detection of the preC mutation and that the absence of serum HBeAg cannot be explained solely by the dominance of the preC mutant.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 009241122

Download citation: RISBibTeXText

PMID: 7595419

DOI: 10.1002/jmv.1890460417


Related references

Prevalence of precore-defective mutant of hepatitis B virus in patients with hepatitis B in Shenyang. Journal of China Medical University 25(5): 505-507, 510, 1996

The prevalence of hepatitis B virus precore mutant isolated from asymptomatic carriers in Guangxi. Zhonghua Liu Xing Bing Xue Za Zhi 23(6): 453-456, 2002

Mutation site-specific assay (MSSA) for the detection of hepatitis B virus precore defective mutant in hepatitis B infection. Nihon Rinsho. Japanese Journal of Clinical Medicine 53 Suppl(Pt 2): 321-324, 1995

Efficacy of lamivudine in patients with hepatitis B e antigen-negative/hepatitis B virus DNA-positive (precore mutant) chronic hepatitis B. Lamivudine Precore Mutant Study Group. Hepatology 29(3): 889-896, 1999

Quantitative analysis of wild-type and precore mutant hepatitis B virus in carriers. Hepatology Research 23(1): 48-54, 2002

Prevalence of precore and core promoter mutations in Hepatitis B e antigen-negative chronic Hepatitis B virus carriers in Korea. Hepatology 34(4 Pt 2): 611A, 2001

A precore-defective mutant of hepatitis B virus associated with e antigen-negative chronic liver disease. Journal of Medical Virology 32(2): 109-118, 1990

Prevalence of precore mutants in anti-HBe-positive hepatitis B virus carriers in Germany. Journal of Medical Virology 59(1): 14-18, 1999

Detection of hepatitis B virus precore stop codon mutants by selective amplification method: Frequent detection of precore mutants in hepatitis B e antigen positive healthy carriers. Journal of Gastroenterology and Hepatology 10(4): 419-425, 1995

Different prevalence of precore mutants in five members of a hepatitis-B-virus-infected family: evidence for a precore variant type in an asymptomatic anti-HBs patient. Journal of Hepatology 21(5): 797-805, 1994