Section 10
Chapter 9,248

Probes for the study of mupirocin resistance in staphylococci

Rahman, M.; Noble, W.C.; Dyke, K.G.

Journal of Medical Microbiology 39(6): 446-449


ISSN/ISBN: 0022-2615
PMID: 8246263
DOI: 10.1099/00222615-39-6-446
Accession: 009247321

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Probes constructed from a 4.05-kb EcoRI digest fragment of a mupirocin resistance plasmid and a 751-bp internal part of this fragment hybridised with DNA from all of 36 independent high-level mupirocin-resistant staphylococci tested from seven centres; most were Staphylococcus aureus. In most instances the probes detected as EcoRI digest fragment of approximately 4 kb. Probes did not hybridise to DNA from low-level resistant strains, nor from strains sensitive to mupirocin.

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