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Production of the new antimalarial drug artemisinin in shoot cultures of Artemisia annua L

, : Production of the new antimalarial drug artemisinin in shoot cultures of Artemisia annua L. Plant Cell Tissue & Organ Culture 32(2): 247-257

From aseptically grown Artemisia annua plantlets, shoot cultures were initiated. Using different concentrations of auxine, cytokine and sucrose, a suitable culture medium was developed, with respect to the growth of the shoots and their artemisinin accumulation. Nitrate concentration and conductivity appeared to be suitable growth parameters. The artemisinin content was measured gas chromatographically. The shoot cultures were maintained in the developed standard medium, consisting of a half concentration of MS-salts with vitamins, 0.2 l-1 BAP, 0.05 mg l-1 NAA and 1% sucrose. The growth of the shoots and the artemisinin content remained stable for a longer period. They showed considerable photosynthetic activity and generally contained ca. 0.08% artemisinin on a dry weight basis. The highest artemisinin content found was 0.16% in the above mentioned standard medium, but also on the same medium with 0.5% sucrose. Attempts were made to further improve the artemisinin production by varying the medium composition through addition of gibberellic acid or casein hydrolysate; by omitting plant growth regulators; by precursor feeding, i.e. mevalonic acid; by influencing the biosynthesis routing through inhibition of the sterol synthesis by miconazole, naftifine or terbinafine; by changing gene expression with 5-azacytidine or colchicine; and by elicitation, using cellulase, chitosan, glutathione or nigeran. Enhanced artemisinin production was found with 10 mg l-1 gibberellic acid, 0.5 g l-1 casein hydrolysate, 10 mg l-1 or 20 mg l-1 naftifine. Relative increases of 154%, 169%, 140% and 120% were found, respectively. Other additions caused the growth to cease and the artemisinin contents to drop.

Accession: 009251921

DOI: 10.1007/bf00029850

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Related references

Woerdenbag, H.J.; Luers, J.F.L.; Van Uden, W.; Pras, N.; Malingre, T.M.; Alfermann, A.W., 1992: Production of the new antimalarial drug artemisinin in shoot cultures of Artemisia annua. Planta Medica 58(7 SUPPL 1): A620-A621

Woerdenbag, H.J.; Luers, J.F.J.; Uden, W. van; Pras, N.; Malingre, T.M.; Alfermann, A.W., 1993: Production of the new antimalaria drug artemisinin in shoot cultures of Artemisia annua L. A suitable culture medium was developed for establishing shoot cultures of A. annua in which significant artemisinin [qinghaosu] concentrations were accumulated. This medium consisted of half strength MS salts with vitamins, 0.2 mg BA and 0.05 mg...

Liu ChunZhao; Guo Chen; Wang YuChun; Fan OuYang, 2003: Factors influencing artemisinin production from shoot cultures of Artemisia annua L. Artemisinin, an anti-malarial drug isolated from the annual wormwood Artemisia annua L., has a marked activity against chloroquine-resistant and chloroquine-sensitive strains of Plasmodium falciparum, and is useful in treatment of cerebral malaria...

Segura, V., 2010: Genetic and breeding of Artemisia annua L. for a sustainable production of the antimalarial drug artemisinin. Medecine Sciences 26(8-9): 701-703

Ferreira, J.; Janick, J., 1996: Roots as an enhancing factor for the production of artemisinin in shoot cultures of Artemisia annua. Artemisinin was produced in differentiated shoot cultures of Artemisia annua L. but was undetected in callus or cell cultures. The growth regulators benzyladenine, kinetin, chlormequat, and daminozide, at concentrations which severely reduced root...

Liu,; Wang, Y.C.un; Guo, C.; Ouyang, F.; Ye, H.C.un; Li,, 1998: Production of artemisinin by shoot cultures of Artemisia annua L. In a modified inner-loop mist bioreactor. A modified inner-loop ultrasonic nutrient mist bioreactor with three stainless steel meshes was used to culture shoots of Artemisia annua L. for the production of artemisinin. The distribution of nutrient mist and the characteristics of shoot grow...

Rodrigues, R.A.F.; Foglio, M.A.; Boaventura Junior, S.; Santos, A. da S.; Rehder, V.L.G., 2006: Optimization of the extraction and isolation of the antimalarial drug artemisinin from Artemisia annua L. Malaria is still one of the major diseases in the world, causing physical and economic problems in tropical regions. Artemisinin (Qinghaosu), a natural compound identified in A. annua, is an effective drug mainly against cerebral malaria. The acti...

Abdin, M.Z.; Israr, M.; Srivastava, P.S.; Jain, S.K., 2001: In vitro production of artemisinin, a novel antimalarial compound from Artemisia annua. Artemisinin, a non-conventional and highly potent antimalarial drug, is extracted from A. annua. It is a sesquiterpenoid lactone with an endoperoxide bridge and is highly effective even in drug-resistant malarial parasites as well as in cerebral m...

Weathers, P.; Smith, T.; Hemmavanh, D.; Follansbee, E.; Ryan, J.; Cheetham, R., 1996: Production of the antimalarial, artemisinin, by transformed roots of Artemisia annua. The effects of varying the strength of B5 culture media, the source and level of nitrogen used (nitrate and ammonium), and phosphate and GA3 in the medium, on both biomass and artemisinin production in hairy root cultures were investigated. Prelim...

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