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Propagation of Eucharis grandiflora Planch: By tissue culture

Propagation of Eucharis grandiflora Planch: By tissue culture

Kagawa Daigaku Nogakubu Gakujutsu Hokoku 47(1): 79-86

Propagation of Eucharis grandiflora by tissue culture and effect of temperature and day length on growing of its young plant during winter of low temperature were investigated in order to get its year-round production. 1. Linsmaier and Skoog (LS) culture medium of pH 5.7-5.8 was prepared with inorganic substances, BA 1 ppm, NAA 0.1 ppm, sucrose 45 g, gellan gum 2 g. Callus or adventitious bud was formed from several kinds of tissue derived from young flower bud by above culture. 2. The several kinds of tissue cultured were such as flower stalk, upper part of flower stalk, bractlet, pedicel and ovary with perianth. Among them, pedicel gave the most superior result. 3. Formation and growth of adventitious bud were affected by a liquid culture with the concentration of BA. With BA 0.1-5 ppm and NAA 0.1 ppm, many of big adventitious buds were formed. 4. Number of shoots and roots was affected by concentration of the total inorganic substances. Three concentrations of 1, 1/2, and 1/5 were examined. Decreased concentration gave decreased number of them. 5. When the first culture was performed by a liquid medium, corpulence of explant was more obvious than by a solid medium. Formation of callus was depressed but many of small adventitious buds were formed on pedicel and the base of bractlet. 6. Acclimatized young plants by the culture were transplanted to plastic pots of 9 cm diameter with mixed soils of equal amount of leaf mold, Kanuma soil and vermiculite. They were grown in six different environments of phytotron rooms of 15 degree C, 20 degree C and 25 degree C, a heated room of over 17 degree C for acclimation under two conditions of natural day length and 15 hours day length, and unheated glass house. They were grown in such environments for 5 months from November to April of the next year. Effects of temperature and day length on growth of young plants during cold winter were investigated. In the room of 15 degree C and the unheated glass house, the growth was inferior. Higher the temperature, greater the number of leaves and the surface area of leaves were. The results obtained in the room for acclimation (at 17 degree C) and the room of 20 degree C were almost the same and a slight increased number of leaves was found with the longer day length than with the natural day length.

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